|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_077305, 20149675|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||26697 Da|
|Application Notes||EFHD2 antibody can be used for detection of EFHD2 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||EFHD2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||EFHD2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May regulate B-cell receptor (BCR)-induced immature and primary B-cell apoptosis. Plays a role as negative regulator of the canonical NF-kappa-B-activating branch. Controls spontaneous apoptosis through the regulation of BCL2L1 abundance.|
|Cellular Location||Membrane raft. Note=In a mouse immature B-cell line WEHI-231.|
|Tissue Location||Found in lymphocytes; preferentially expressed in CD8+ cells.|
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Provided below are standard protocols that you may find useful for product applications.
EFHD2 Antibody: EFHD2, also known as Swiprosin-1 or SWS1, is an EF-hand and coiled-coil-containing adaptor protein that plays a role in lymphocyte physiology. EFHD2 exhibits the highest expression in CD8+ T cells and immature B cells. It provides a membrane scaffold that is required for the Syk-, SLP-65-, and PLCgamma2-dependent B-cell receptor (BCR)-induced calcium flux. EFHD2 may also regulate BCR-induced immature and primary B-cell apoptosis. It controls spontaneous apoptosis through the regulation of BCL2L1 abundance. Also, EFHD2 plays a role as negative regulator of the canonical NF-κB-activating branch.
Kroczek C, Lang C, Brachs S, et al. Swiprosin-1/EFhd2 controls B cell receptor signaling through the assembly of the B cell receptor, Syk, and phospholipase C gamma2 in membrane rafts. J. Immunol.2010; 184:3665-76.
Avramidou A, Kroczek C, Lang C, et al. The novel adaptor protein Swiprosin-1 enhances BCR signals and contributes to BCR-induced apoptosis. Cell Death Differ.2007; 14:1936-47.
Thylur RP, Kiim YD, Kwon MS, et al. Swiprosin-1 is expressed in mast cells and up-regulated through the protein kinase C beta I/eta pathway. J. Cell Biochem.2009; 108:705-15.
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