|Application ||WB, IHC-P, IF, E|
|Other Accession||EAW83167, 119603573|
|Calculated MW||67257 Da|
|Application Notes||RANBP10 antibody can be used for detection of RANBP10 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||RANBP10 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||RANBP10 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as a guanine nucleotide exchange factor (GEF) for RAN GTPase (By similarity). May play an essential role in hemostasis and in maintaining microtubule dynamics with respect to both platelet shape and function (By similarity). May act as an adapter protein to couple membrane receptors to intracellular signaling pathways. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen- induced transactivation. In contrast to RANBP9, does not interact with Sos and does not activate the Ras pathway.|
|Cellular Location||Cytoplasm, cytosol. Nucleus. Note=Predominantly cytoplasmic|
|Tissue Location||Broadly expressed, with highest levels in skeletal muscle.|
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Provided below are standard protocols that you may find useful for product applications.
RANBP10 Antibody: The RAN binding protein 10 (RANBP10) is a cytoplasmic guanine nucleotide exchange factor that shares high sequence similarity to the MET-interacting protein RANBP9. RANBP10 is expressed in the cytoplasm of mature megakaryocytes (MKs) and platelets where it localizes to polymerized noncentrosomal microtubules. RANBP10 possesses guanine nucleotide exchange factor activity towards RAN, a GTPase involved in nucleocytoplasmic traffic, spindle formation at mitosis, and nuclear envelope assembly during telophase, suggesting that RANBP10 may also be involved in these activities. RANBP10 depletion in vitro caused the disturbance of polymerized filaments in MKs and platelets in RANBP10-null mice exhibited disorders in microtubule filament numbers and localization, indicating that RANBP10 may play a significant role in maintaining microtubule dynamics.
Wang D, Zaibo Li, Schoen SR, et al. A novel MET-interacting protein shares high sequence similarity with RanBPM, but fails to stimulate MET-induced Ras/ERK signaling. Biochem. Biophys. Res. Comm.2004; 313:320-6.
Schulze H, Dose M, Korpal M, et al. RanBP10 is a cytoplasmic guanine nucleotide exchange factor that modulates noncentrosomal microtubules. J. Biol. Chem.2008; 283:14109-19.
Dasso M. The Ran GTPase: theme and variations. Curr. Biol.2002; R502-8.
Kunert S, Meyer I, Fleischhauer S, et al. The microtubule modulator RanBP10 plays a critical role in regulation of platelet discoid shape and degranulation. Blood2009; 114:5532-40.
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