|Application ||WB, E|
|Other Accession||EAW47801, 119568186|
|Calculated MW||Predicted: 58 kDa |
Observed: 57 kDa
|Application Notes||ZC3H12D antibody can be used for detection of ZC3H12D by Western blot at 1 µg/mL.|
|Reconstitution & Storage||ZC3H12D antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||ZC3H12D Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May regulate cell growth likely by suppressing RB1 phosphorylation (PubMed:19531561). May function as RNase and regulate the levels of target RNA species (Potential). In association with ZC3H12A enhances the degradation of interleukin IL-6 mRNA level in activated macrophages (PubMed:26134560). Serve as a tumor suppressor in certain leukemia cells (PubMed:17210687). Overexpression inhibits the G1 to S phase progression through suppression of RB1 phosphorylation (PubMed:19531561).|
|Cellular Location||Cytoplasm, P-body. Note=Colocalizes with ZC3H12A in GW bodies (GWBs) (PubMed:26134560). Isoform 3: Cytoplasm. Nucleus.|
|Tissue Location||Expressed in normal human lymphocytes but defective in some leukemia/lymphoma cell lines|
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Provided below are standard protocols that you may find useful for product applications.
ZC3H12D Antibody: ZC3H12D, also known as MCPIP4, is a member of a family of novel CCCH-zinc finger proteins that includes ZC3H12A, a protein that is thought to be involved in macrophage activation, host immunity and inflammatory diseases. ZC3H12D is highly expressed in spleen, intestine, and colon. Similar to ZC3H12A and ZC3H12C, ZC3H12D expression in murine macrophages is also increased after treatment with lipopolysaccharide (LPS), although not to the same level as ZC3H12A. This pattern of expression was not duplicated in human-derived macrophages, suggesting it also may play a different role in host immunity and inflammatory response.
Zhou L, Azfer A, Niu J, et al. Monocyte chemoattractant protein-1 induces a novel transcription factor that causes cardiac myocyte apoptosis and ventricular dysfunction. Circ. Res.2006; 98:1177-85.
Liang J, Wang J, Azfer A, et al. A novel CCCH-zinc finger protein family regulates proinflammatory activation of macrophages. J. Biol. Chem.2008; 283:6337-46.
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