|Application ||WB, IF, ICC, E|
|Other Accession||AAV51405, 55418570|
|Calculated MW||65923 Da|
|Application Notes||ENC-2 antibody can be used for detection of ENC-2 by Western blot at 1 µg/mL. Antibody can also be used for immunocytochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||ENC-2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||ENC-2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for translational homeostasis. The BCR(KLHL25) ubiquitin ligase complex acts by mediating ubiquitination of hypophosphorylated EIF4EBP1 (4E-BP1): ubiquitination and subsequent degradation of hypophosphorylated EIF4EBP1 (4E-BP1) probably serves as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. The BCR(KLHL25) complex does not target EIF4EBP1 (4E-BP1) when it is hyperphosphorylated or associated with eIF4E.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
ENC-2 Antibody: The ectoderm-neural cortex-2 (ENC-2) protein a member of the BTB/PDZ Kelch protein family displaying significant homology to ENC-1, an actin-binding protein involved in neural fate specification during embryogenesis and adipocyte maturation. The exact function of ENC-2 has yet to be determined, but it has been suggested to be involved in the differentiation of neuronal precursor cells into neurons and astrocytes.
Hernandez MC, Andres-Barquin PJ, Martinez S, et al. ENC-1: a novel mammalian kelch-related gene specifically expressed in the nervous system encodes an actin-binding protein. J. Neurosci.1997; 17:3038-51.
Zhao L, Gregoire F and Sul HS. Transient induction of ENC-1, a Kelch-related actin-binding protein, is required for adipocyte differentiation. J. Biol. Chem.2000; 275:16845-50.
Ahn JI, Kim SY, Ko MJ, et al. Analysis of gene expression in mouse spinal cord-derived neural precursor cells during neuronal differentiation. Gen. and Infor.2009; 7:85-96.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.