|Application ||WB, IF, ICC, E|
|Other Accession||NP_653314, 283046686|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||47399 Da|
|Application Notes||FAM82A1 antibody can be used for detection of FAM82A1 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunocytochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||FAM82A1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||FAM82A1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Cellular Location||Membrane; Single-pass membrane protein. Cytoplasm Cytoplasm, cytoskeleton, spindle Cytoplasm, cytoskeleton, spindle pole. Note=In interphase localizes in the cytoplasm, and during mitosis localizes to the spindle microtubules and spindle poles. Also detected as large dots in the perinuclear region|
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Provided below are standard protocols that you may find useful for product applications.
FAM82A1 Antibody: The human protein FAM82A1, also known as regulator of microtubule dynamics 2 (RMD2), was one of three proteins identified as related to a family of microtubule-associated proteins in C. elegans. FAM82A1 contains multiple coiled-coil domains and localize to the spindle microtubules and spindle poles during cell division. During interphase, RMD2 localizes to the cytoplasm with protein observed in the microtubule lattice and perinuclear region.
Oishi K, Okanu H, and Sawa H. RMD-1, a novel microtubule-associated protein, functions in chromosome segregation in Caenorhabditis elegans. J. Cell Biol.2007; 179:1149-62.
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