|Application ||WB, IF, E|
|Other Accession||NP_001002244, 51529|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||9841 Da|
|Application Notes||APC11 antibody can be used for detection of APC11 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.|
|Target/Specificity||APC11 antibody was raised against an 18 amino acid synthetic peptide near the center of human APC11.|
The immunogen is located within amino acids 70 - 120 of APC11.
|Reconstitution & Storage||APC11 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||APC11 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Together with the cullin protein ANAPC2, constitutes the catalytic component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. May recruit the E2 ubiquitin-conjugating enzymes to the complex.|
|Cellular Location||Cytoplasm. Nucleus|
|Tissue Location||Expressed at high levels in skeletal muscle and heart; in moderate levels in brain, kidney, and liver; and at low levels in colon, thymus, spleen, small intestine, placenta, lung and peripheral blood leukocyte|
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Provided below are standard protocols that you may find useful for product applications.
APC11 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC11 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. APC11, together with APC2 and Ubc4, form the minimal APC ubiquitin ligase module.
JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56.
Jorgensen PM, Graslund S, Betz R, et al. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene2001; 262:51-9.
Tan Z, Li B, Bharadwaj R, et al. APC Cullin protein and APC RING protein comprise the minimal ubiquitin ligase module of the anaphase-promoting complex. Mol. Biol. Cell2001; 12:3839-51.
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