|Application ||WB, IF, E|
|Other Accession||NP_056206, 25847|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||8521 Da|
|Application Notes||APC13 antibody can be used for detection of APC13 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.|
|Target/Specificity||APC13 antibody was raised against a 17 amino acid synthetic peptide near the center of human APC13.|
The immunogen is located within the last 50 amino acids of APC13.
|Reconstitution & Storage||APC13 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||APC13 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.|
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Provided below are standard protocols that you may find useful for product applications.
APC13 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC13 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. APC13 promotes the stable association of APC3/Cdc27 and APC6/Cdc16 with the APC/C.
JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56.
Jorgensen PM, Graslund S, Betz R, et al. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene2001; 262:51-9.
Schwickart M, Havlis J, Habermann B, et al. Swm1/Apc13 is an evolutionarily conserved subunit of the anaphase-promoting complex stabilizing the association of Cdc16 and Cdc27. Mol. Cell. Biol.2004; 24:3562-76.
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