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SAE2 Antibody

  • WB - SAE2 Antibody ASC11129
    Western blot analysis of SAE2 in 293 cell lysate with SAE2 antibody at 0.25 µg/mL in (A) the absence and (B) the presence of blocking peptide.
  • ICC - SAE2 Antibody ASC11129
    Immunocytochemistry of SAE2 in 293 cells with SAE2 antibody at 4 µg/mL.
  • IF - SAE2 Antibody ASC11129
    Immunofluorescence of SAE2 in 293 cells with SAE2 antibody at 20 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UBT2
Other Accession NP_005490, 4885649
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 71224 Da
Application Notes SAE2 antibody can be used for detection of SAE2 by Western blot at 0.25 µg/mL. Antibody can also be used for immunocytochemistry starting at 4 µg/mL. For immunofluorescence start at 5 µg/mL.
Additional Information
Gene ID 10054
Target/Specificity UBA2;
Reconstitution & Storage SAE2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsSAE2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UBA2
Synonyms SAE2, UBLE1B
Function The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Cellular Location Cytoplasm. Nucleus. Note=Shuttles between the cytoplasm and the nucleus, sumoylation is required either for nuclear translocation or nuclear retention
Research Areas
Citations (0)

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SAE2 Antibody: Small ubiquitin-like modifiers (SUMOs) are a family of small, related proteins (SUMO-1/2/3/4) that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation, a major regulator of protein function in cellular processes such as nuclear transport, transcriptional regulation, apoptosis and protein stability. This sumoylation is effected by the heterodimeric enzyme SAE1/SAE2 and the SUMO-1-conjugating enzyme Ubch9. The sumoylation pathway mediated by SAE1/SAE2 is distinct from other ubiquitin-like protein (Ubl) pathways.


Kamitani T, Kito K, Nguyen HP, et al. Characterization of a second member of the sentrin family of ubiquitin-like proteins. J. Biol. Chem.1998;273:11349-53.
Kim KI, Baek SH, and Chung CH. Versatile protein tag, SUMO: its enzymology and biological function. J. Cell. Physiol.2002; 191: 257-68.
Desterro JM, Rodriguez MS, Kemp GD, et al. Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1. J. Biol. Chem.1999; 274:10618-24.
Tatham MH, Jaffray E, Vaughan OA, et al. Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9. J. Biol. Chem.2001; 276:35368-74.

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$ 310.00
Cat# ASC11129
(40 western blots)
Availability: 5-7days
Bulk Size
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