|Application ||WB, E|
|Other Accession||NP_775956, 27734761|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 27 kDa |
Observed: 30 kDa
|Application Notes||MMS21 antibody can be used for detection of MMS21 by Western blot at 0.5 - 1 µg/mL.|
|Reconstitution & Storage||MMS21 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||MMS21 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand break repair by homologous recombination. Is not be required for the stability of the complex. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, the shelterin complex subunits TERF1, TERF2, TINF2 and TERF2IP, and maybe the cohesin components RAD21 and STAG2. Required for recruitment of telomeres to PML nuclear bodies. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair, and for formation of APBs in ALT cell lines. Required for sister chromatid cohesion during prometaphase and mitotic progression.|
|Cellular Location||Nucleus. Chromosome, telomere. Note=Localizes to PML nuclear bodies in ALT cell lines|
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Provided below are standard protocols that you may find useful for product applications.
MMS21 Antibody: MMS21, also known as NSE2, is a SUMO ligase that in combination with the SMC5/6 complex is required for the prevention of DNA damage induced apoptosis by facilitating DNA repair in human cells. MMS21-dependent sumoylation is integral and important to the cohesion mechanism and mitotic progression; this function appears to be independent of SMC6. MMS21 mediates SUMO attachment to various proteins such as SMC6L1 and TRAX, and possibly the cohesin components RAD21 and STAG2.
Potts PR and Yu H. Human MMS21/NSE2 is a SUMO ligase required for DNA repair. Mol. Cell. Biol. 2005; 25:7021-32.
Behlke-Steinert S, Touat-Todeschini L, Skoufias DA, et al. SMC5 and MMS21 are required for chromosome cohesion and mitotic progression. Cell Cycle 2009; 8:2211-8.
Bermudez-Lopez M, Ceschia A, de Piccoli G, et al. The Smc5/6 complex is required for dissolution of DNA-mediated sister chromatid linkages. Nucleic Acids Res. 2010; 38:6502-12.
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