|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_976037, 42718017|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||101942 Da|
|Application Notes||RBBP8 antibody can be used for detection of RBBP8 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||RBBP8 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||RBBP8 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in DNA-end resection, the first step of double- strand break (DSB) repair through the homologous recombination (HR) pathway. HR is restricted to S and G2 phases of the cell cycle and preferentially repairs DSBs resulting from replication fork collapse. Key determinant of DSB repair pathway choice, as it commits cells to HR by preventing classical non-homologous end- joining (NHEJ). Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage (PubMed:10764811, PubMed:10910365, PubMed:15485915, PubMed:16581787, PubMed:16818604, PubMed:17965729, PubMed:19202191, PubMed:19759395, PubMed:20064462, PubMed:20829486). During immunoglobulin heavy chain class-switch recombination, promotes microhomology-mediated alternative end joining (A-NHEJ) and plays an essential role in chromosomal translocations (By similarity).|
|Cellular Location||Nucleus Chromosome. Note=Associates with sites of DNA damage in S/G2 phase (PubMed:10764811, Ref.29). Ubiquitinated RBBP8 binds to chromatin following DNA damage (PubMed:16818604)|
|Tissue Location||Expressed in ER-positive breast cancer lines, but tends to be down-regulated ER-negative cells (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
RBBP8 Antibody: RBBP8, also known as CtBP (carboxy-terminal binding protein) interacting protein (CTIP), was characterized for its role in transcription as a cofactor for the transcriptional repressor CtBP, and also as a binding partner for other proteins including the cell cycle regulators retinoblastoma protein (Rb) and breast cancer 1 (BRCA1). It is ubiquitously expressed and localizes to the nucleus. RBBP8 is thought to modulate the functions in cell proliferation, transcriptional regulation and DNA repair. RBBP8 also plays a central role in the cell cycle checkpoint response to DNA double-stranded breaks (DSBs), with new evidence demonstrating that it controls the choice of DSB repair pathway.
Yu X, Wu LC, Bowcock AM, et al. The C-terminal (BRCT) domains of BRCA1 interact in vivo with CtIP, a protein implicated in the CtBP pathway of transcriptional repression. J. Biol. Chem.1998; 273:25388-92.
Liu F and Lee WH. CtIP activates its own and cyclin D1 promoters via the E2F/RB pathway during G1/S progression. Mol. Cell Biol.2006; 26:3124-34.
Sterner JM, Dew-Knight S, Musahl C, et al. Negative regulation of DNA replication by the retinoblastoma protein is mediated by its association with MCM7. Mol. Cell Biol.1998; 18:2748-57.
Wu G and Lee WH. CtIP, a multivalent adaptor connecting transcriptional regulation, checkpoint control and tumor suppression. Cell Cycle2006; 5:1592-6.
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