|Application ||IHC-P, E|
|Other Accession||NP_689966, 31377568|
|Calculated MW||Predicted: 109 kDa|
|Application Notes||SCUBE3 antibody can be used for detection of SCUBE3 by immunohistochemistry at 5 µg/mL.|
|Reconstitution & Storage||SCUBE3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||SCUBE3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds to TGFBR2 and activates TGFB signaling. In lung cancer cells, could serve as an endogenous autocrine and paracrine ligand of TGFBR2, which could regulate TGFBR2 signaling and hence modulate epithelial-mesenchymal transition and cancer progression.|
|Cellular Location||Secreted. Cell surface|
|Tissue Location||Highly expressed in osteoblasts. In normal lung, mainly expressed in bronchial epithelial cells. Tends to be up-regulated in lung cancer cells.|
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Provided below are standard protocols that you may find useful for product applications.
SCUBE3 Antibody: SCUBE3 is a member of a family of secreted glycoproteins that contain N-terminal EGF-like repeats and C-terminal cysteine-rich motifs and CUB domain and is highly expressed in primary osteoblasts and bones, and to a lesser extent in heart (1,2). Other studies have shown that overexpression of SCUBE3 in mice induced cardiac hypertrophy, suggesting that it may also play a role in the regulation of cardiac growth. SCUBE3 has been shown to be an endogenous TGF-beta receptor ligand (3,4) and is thought to promote lung cancer cell mobility and invasiveness. In lung cancer cells, the secreted SCUBE3 protein was cleaved by MMP2 and MMP9, allowing the activation of the TGF-beta receptor, the increase of Smad2/3 transcriptional activity and the upregulation of expression of proteins such as TGF-beta1, VEGF, Snail, and Slug.
Wu BT, Su YH, Tsai MT, et al. A novel secreted, cell-surface glycoprotein containing multiple epidermal growth factor-like repeats and one CUB domain is highly expressed in primary osteoblasts and bones. J. Biol. Chem. 2004; 279:37485-90.
Yang RB, Ng CK, Wasserman SM, et al. Identification of a novel family of cell-surface proteins expressed in human vascular endothelium. J. Biol. Chem. 2002; 277:46364-73.
Yang HY, Cheng CF, Djoko B, et al. Transgenic overexpression of the secreted, extracellular EGF-CUB domain containing protein SCUBE3 induces cardiac hypertrophy in mice. Cardiovas. Res. 2007; 75:139-47.
Wu YY, Peck K, Chang YL, et al. SCUBE3 is an endogenous TGF- receptor ligand and regulates the epithelial-mesenchymal transition in lung cancer. Oncogene 2011; 30:3682-93.
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