|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_037433, 94536856|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||32032 Da|
|Application Notes||CCDC106 antibody can be used for detection of CCDC106 by Western blot at 0.5 - 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||CCDC106 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||CCDC106 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Promotes the degradation of p53/TP53 protein and inhibits its transactivity.|
|Cellular Location||Nucleus. Note=Colocalizes with p53/TP53|
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Provided below are standard protocols that you may find useful for product applications.
CCDC106 Antibody: The coiled-coil domain is a common protein motif that is often involved in protein oligomerization and is found in proteins such as transcription factors and intermediate filaments. CCDC106 was initially identified as a p53-interacting protein by yeast two-hybrid screening. Other experiments demonstrated that CCDC106 co-localizes and interacts with p53 in the nucleus, inhibiting the transcriptional activity of p53 and stimulating p53 protein degradation, indicating that at least one of the functions of CCDC106 is acting as a negative regulator of p53.
Steinmetz MO, Jelesarov I, Matousek WM, et al. Molecular basis of coiled-coil formation. Proc. Natl. Acad. Sci. USA2007; 104:7062-7.
telzl U, Worm U, Lalowski M, et al. A human protein-protein interaction network: a resource for annotating the proteome. Cell2005; 122:957-68.
Zhou J, Qiao X, Xiao L, et al. Identification and characterization of the novel protein CCDC106 that interacts with p53 and promotes its degradation. FEBS Lett.2010; 584:1085-90.
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