Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
COVID19
>   home   >   Products   >   Primary Antibodies   >   Developmental Biology   >   CRISP2 Antibody   

CRISP2 Antibody

     
  • WB - CRISP2 Antibody ASC11180
    Western blot analysis of CRISP2 in human testis tissue lysate with CRISP2 antibody at (A) 0.5 and (B) 1 µg/mL.
    detail
  • IHC - CRISP2 Antibody ASC11180
    Immunohistochemistry of CRISP2 in human testis tissue with CRISP2 antibody at 10 µg/mL.
    detail
  • IF - CRISP2 Antibody ASC11180
    Immunofluorescence of CRISP in human testis tissue with CRISP antibody at 20 µg/mL.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession P16562
Other Accession NP_001135880, 215422434
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 27259 Da
Application Notes CRISP2 antibody can be used for detection of CRISP2 by Western blot at 0.5 - 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 10 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 7180
Target/Specificity CRISP2;
Reconstitution & Storage CRISP2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsCRISP2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CRISP2
Synonyms GAPDL5, TPX1
Function May regulate some ion channels' activity and therebye regulate calcium fluxes during sperm capacitation.
Cellular Location Secreted.
Tissue Location Testis and epididymis.
Research Areas
Citations (0)
citation

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abcepta antibody to
info@abcepta.com, and receive a free "I Love Antibodies" mug.

Background

CRISP2 Antibody: The cysteine-rich secretory proteins (CRISP) family is a group of four proteins that are strongly expressed in the male reproductive tract and have been implicated in having roles in male fertility. CRISP2, also known as TPX1, has been implicated in the adhesion between spermatids and Sertoli cells, and with CRISP1, is thought to be involved in sperm-egg fusion. CRISP2 has been shown to regulate the Ca2+ influx through ryanodine receptors (RYR) and may influence the acrosome reaction or sperm motility. CRISP2 has also been shown to bind to the mitogen-activated protein kinase kinase kinase 11 (MAP3K11) and localizes to the developing acrosome, suggesting this CRISP2-MAP3K11 complex may have a role in acrosome development.

References

Cohen DJ, Busso D, Da Ros V, et al. Participation of cysteine-rich secretory proteins (CRISP) in mammalian sperm-egg interaction. Int. J. Dev. Biol.2008; 52:737-42.
Maeda T, Sakashita M, Ohba Y, et al. Molecular cloning of the rat Tpx-1 responsible for the interaction between spermatogenic and Sertoli cells. Biochem. Biophys. Res. Commun.1998; 248:140-6.
Busso D, Goldweic NM, Hayashi M, et al. Evidence for the involvement of testicular protein CRISP2 in mouse sperm-egg fusion. Biol. Reprod.2007; 76:701-8.
Gibbs GM, Scanlon MJ, Swarbrick J, et al. The cysteine-rich secretory protein domain of Tpx-1 is related to ion channel toxins and regulates ryanodine receptor Ca2+ signaling. J. Biol. Chem.2006; 281:4156-63.

FeedBack
Abcepta welcomes feedback from its customers.

If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abcepta.com.

$ 379.00
Cat# ASC11180
Size:
Quantity:
Availability: 5-7days
Bulk Size

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaCentral AmericaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSouth KoreaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamWorldwideOthers
Abcepta, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Terms & Conditions
"