|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_115745, 91992155|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||31683 Da|
|Application Notes||HVCN1 antibody can be used for detection of HVCN1 by Western blot at 0.5 - 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||HVCN1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||HVCN1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Mediates the voltage-dependent proton permeability of excitable membranes. Forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis.|
|Cellular Location||Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note=Detected mainly at intracellular membranes upon overexpression in HeLa cells (PuMed:20147290), but not in other cell types|
|Tissue Location||Enriched in immune tissues, such as lymph nodes, B-lymphocytes, monocytes and spleen|
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Provided below are standard protocols that you may find useful for product applications.
HVCN1 Antibody: HVCN1 (hydrogen voltage-gated channel 1), a voltage gated proton channel protein that is highly expressed in immune tissues, mediates the proton conductances required by phagocytic leukocytes for the oxidative burst that underlies microbial killing. HVCN1 moderates the voltage-dependent proton permeability of excitable membranes by allowing the flow of protons in accordance to their electrochemical gradient. HVCN1 is sensitive to zinc ions, and can be inhibited by them. HVCN1 may represent a new therapeutic target for B cell malignancies on continued signaling via the B cell antigen receptor.
Ramsey IS, Moran MM, Chong JA, et al. A voltage gated proton-selective channel lacking the pore domain. Nature2006; 440:1213-6.
Sasaki M, Takagi M, and Okamura Y. A voltage sensor-domain protein is a voltage-gated proton channel. Science2006; 312: 589-92.
Morgan D, Capasso M, and Musset B. Voltage-gated proton channels maintain pH in human neutrophils during phagocytosis. Proc. Natl. Acad. Sci. USA2009; 106:18022-7.
Capasso M, Bhamrah MK, Henley T, et al. HVCN1 modulates BCR signal strength via regulation of BCR-dependent generation of reactive oxygen species. Nat. Immunol.2010;11:265-72.
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