|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_057066, 156415990|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||38518 Da|
|Application Notes||YBX2 antibody can be used for detection of YBX2 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 10 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||YBX2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||YBX2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Major constituent of messenger ribonucleoprotein particles (mRNPs). Involved in the regulation of the stability and/or translation of germ cell mRNAs. Binds to Y-box consensus promoter element. Binds to full length mRNA with high affinity in a sequence-independent manner. Binds to short RNA sequences containing the consensus site 5'-UCCAUCA-3' with low affinity and limited sequence specificity. Its binding with maternal mRNAs is necessary for its cytoplasmic retention. May mark specific mRNAs (those transcribed from Y-box promoters) in the nucleus for cytoplasmic storage, thereby linking transcription and mRNA storage/translational delay (By similarity).|
|Cellular Location||Cytoplasm. Nucleus|
|Tissue Location||Expressed in oocytes and testicular germ cells in the stage of spermatogonia to spermatocyte. Also observed placental trophoblasts, as well as in vascular smooth muscle cells in the pulmonary artery, myocardium, and skeletal muscle Undetectable in epithelial cells in respiratory, gastrointestinal, and urogenital tracts. Up-regulated in various carcinomas and germ cell tumors (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
YBX2 Antibody: Germ cell specific Y-box binding protein (YBX2), a germ-cell-specific member of the Y-box family of DNA-/RNA-binding proteins, is a major constituent of messenger ribonucleoprotein particles (mRNPs) and involved in the regulation of the stability and/or translation of germ cell mRNAs. It is proposed to function as a coactivator of transcription in the nucleus and to stabilize and store maternal and paternal mRNAs in the cytoplasm. YBX2 binds to the Y-box consensus promoter element and is expressed in oocytes and testicular germ cells in the stage of spermatogonia to spermatocyte. Recent studies show that deletion of YBX2 leads to the disruption of both spermatogenesis and oogenesis.
Kohno Y, Matsuki Y, Tanimoto A, et al. Expression of Y-box-binding protein dbpC/contrin, a potentially new cancer/testis antigen. Br. J. Cancer2006; 94:710-6.
Medvedev S, Yang J, Hecht NB, et al. CDC2A (CDK1)-mediated phosphorylation of MSY2 triggers maternal mRNA degradation during mouse oocyte maturation. Dev. Biol.2008; 321:205-15.
Yang J, Medvedev S, Yu J, et al. Absence of the DNA-/RNA-binding protein MSY2 results in male and female infertility. Proc. Natl. Acad. Sci. USA2005; 102:5755-60.
Hammoud S, Emery BR, and Dunn D. Sequence alterations in the YBX2 gene are associated with male factor infertility. Fertil. Steril.2009; 91:1090-5.
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