|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_689763, 222446607|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||79320 Da|
|Application Notes||SH3RF2 antibody can be used for detection of SH3RF2 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 10 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||SH3RF2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||SH3RF2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inhibits PPP1CA phosphatase activity. May be a E3 ubiquitin-protein ligase (Potential). May play a role in cardiac function.|
|Tissue Location||Heart (at protein level). Heart and testis. In the heart, present in the apex, left atrium, right atrium, left ventricle and right ventricle, but not in the aorta|
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Provided below are standard protocols that you may find useful for product applications.
SH3RF2 Antibody: Although the SH3 domain containing ring finger 2 protein (SH3RF2) has been identified as a marker of the nucleus accumbens in the human brain, little is known about this protein. Recent yeast two-hybrid experiments have shown that SH3RF2 associates with protein phosphatase 1 (PP1) and is expressed primarily in heart and testis tissue. Recombinant SH3RF2 enhanced PP1 enzymatic activity and antagonized the ability of phopho-inhibitor-1 or -2 to inhibit PP1, suggesting that SH3RF2 may play a role working with PP1 in cardiac functions.
Ernst C, Sequeira A, Klempan, et al. Confirmation of region-specific patterns of gene expression in the human brain. Neurogenetics2007; 8:219-24.
Chen CY, Lai NS, Yang JJ, et al. FLJ23654 encodes a heart protein phosphatase 1-binding protein (Hepp1). Biochem. Biophys. Res. Commun.2010; 391:698-702.
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