|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_078894, 25777608|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||107616 Da|
|Application Notes||NOD5 antibody can be used for detection of NOD5 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 10 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||NOD5 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||NOD5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||NOD26, NOD5, NOD9|
|Function||Participates in antiviral signaling. Acts as a negative regulator of MAVS-mediated antiviral responses, through the inhibition of the virus-induced RLH (RIG-like helicase)-MAVS interaction (PubMed:18200010). Instead, promotes autophagy by interacting with TUFM and subsequently recruiting the autophagy- related proteins ATG5 and ATG12 (PubMed:22749352). Regulates also MAVS-dependent NLRP3 inflammasome activation to attenuate apoptosis (PubMed:27393910). Has no inhibitory function on NF- kappa-B signaling pathway, but enhances NF-kappa-B and JUN N- terminal kinase dependent signaling through the production of reactive oxygen species (PubMed:18219313).|
|Cellular Location||Mitochondrion outer membrane|
|Tissue Location||Ubiquitously expressed. Strongest expression in mammary gland, heart and muscle. Detected in HeLa, HEK293T, THP-1, HL-60, Raji and Jurkat cell lines (at protein level)|
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Provided below are standard protocols that you may find useful for product applications.
NOD5 Antibody: NOD5, also known as NLRX1, is a member of the NOD (nucleotide-binding oligomerization domain) family, a group of proteins that are involved in innate immune defense. NOD5 localizes to the mitochondrial outer membrane and interacts with the virus-induced signaling adapter protein VISA. Unlike a subset of NOD-like receptors (NLRs) such as NOD1 and NOD2 which trigger pro-inflammatory cascades, and other NLRs that induce the caspase 1 inflammasome in response to immune challenges, NOD5 amplifies NF-κB and JNK pathways by inducing reactive oxygen species production.
Kufer TA, Banks DJ, and Philpott DJ. Innate immune sensing of microbes by Nod proteins. Ann. NY Acad. Sci.2006; 1072:19-27.
Moore CB, Bergstralh DT, Duncan JA, et al. NLRX1 is a regulator of mitochondrial antiviral immunity. Nature2008; 451:573-7.
Tattoli I, Carneiro LA, Jehanno M, et al. NLRX1 is a mitochondrial NOD-like receptor that amplifies NF-kB and JNK pathways by inducing reactive oxygen species. EMBO Reports2008; 9:293-300.
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