NALP5 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IF, E |
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Primary Accession | P59047 |
Other Accession | NP_703148, 158321897 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | 134342 Da |
Application Notes | NALP5 antibody can be used for detection of NALP5 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 10 µg/mL. For immunofluorescence start at 20 µg/mL. |
Gene ID | 126206 |
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Target/Specificity | NLRP5; |
Reconstitution & Storage | NALP5 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Precautions | NALP5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | NLRP5 |
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Synonyms | MATER {ECO:0000303|PubMed:19542546}, NAL |
Function | As a member of the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (By similarity). Required for the formation of F-actin cytoplasmic lattices (CPL) in oocytes, which in turn are responsible for symmetric division of zygotes via the regulation of mitotic spindle formation and positioning (By similarity). Required for the localization of cortical granules to the cortex of oocytes, via association with the cortical actin scaffold (By similarity). Required for cortical actin clearance prior to oocyte exocytosis (By similarity). Involved in regulating post-fertilization Ca(2+) release and endoplasmic reticulum (ER) storage via regulation of ER localization (By similarity). May be involved in the localization of mitochondria to the cytoplasm and perinuclear region in oocytes and early stage embryos, independent of its role in CPL formation (By similarity). |
Cellular Location | Cytoplasmic vesicle, secretory vesicle, Cortical granule. Mitochondrion. Nucleus, nucleolus. Cytoplasm. Golgi apparatus |
Tissue Location | Expressed in cumulus cells (at protein level) (PubMed:19542546). Highly abundant in oocytes and early embryos, however poorly expressed in somatic tissues such as the liver and spinal cord (PubMed:11925379, PubMed:30877238) |
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Provided below are standard protocols that you may find useful for product applications.
Background
NALP5 Antibody: NALP proteins include the apoptosis regulator APAF1 (apoptotic protease activating factor 1) and mammalian NOD-LRR proteins and are thought to be involved in inflammation and reproduction. NALP5, also known as MATER, is a maternal gene required for early embryonic development in mice. Increased NALP5 expression was observed in two neuronal injury models, and transient expression of recombinant NALP5 in neurons induced caspase-3 activation and apoptosis, suggesting that NALP5 also plays a role in caspase activation and apoptosis in injured neurons, and may thus represent a novel target for therapeutic treatment in neurodegenerative disorders.
References
Tschopp J, Martinon F, and Burns K. NALPs: a novel protein family involved in inflammation. Nat. Rev. Mol. Cell Biol.2003; 4:95-104.
Tian X, Pascal G, and Monget P. Evolution and functional divergence of NLRP genes in mammalian reproductive system. BMC Evol. Biol.2009; 9:202.
Tong ZB, Gold L, Pfeiffer KE, et al. Mater, a maternal effect gene required for early embryonic development in mice. Nat. Genet.2000; 26:267-8.
Frederick Lo C, Ning X, Gonzales C, et al. Induced expression of death domain genes NALP1 and NALP5 following neuronal injury. Biochem. Biophys. Res. Commun.2008; 366:664-9.
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