|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_653288, 21955154|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||120173 Da|
|Application Notes||NALP12 antibody can be used for detection of NALP12 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||NALP12 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||NALP12 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||NALP12, PYPAF7, RNO|
|Function||May mediate activation of CASP1 via ASC and promote activation of NF-kappa-B via IKK.|
|Tissue Location||Detected only in peripheral blood leukocytes, predominantly in eosinophils and granulocytes, and at lower levels in monocytes.|
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Provided below are standard protocols that you may find useful for product applications.
NALP12 Antibody: NALP proteins are cytoplasmic proteins that form a subfamily within the larger CATERPILLER family and are thought to play a crucial role in cell proliferation and reproduction. Like all other NALP family members, NALP12, also known as Monarch-1, has a C-terminal leucine-rich repeat (LRR) region, an N-terminal Pyrin domain (PYD) followed by a NACHT domain, and a NACHT-associated domain. NALP12 is thought to act as an attenuating factor of inflammation by suppressing inflammatory responses such as NF-κB activation by TLR-signaling molecules MyD88, IRAK-1, TRAF6 and RIPK1 in activated monocytes. Recent evidence suggests that mutations in NALP12 result in hereditary periodic fever syndromes.
Tschopp J, Martinon F, and Burns K. NALPs: a novel protein family involved in inflammation. Nat. Rev. Mol. Cell Biol.2003; 4:95-104.
Tian X, Pascal G, and Monget P. Evolution and functional divergence of NLRP genes in mammalian reproductive system. BMC Evol. Biol.2009; 9:202.
Williams KL, Lich JD, Duncan JA, et al. The CATERPILLER protein Monarch-1 is an antagonist of toll-like receptor- , tumor necrosis factor a-, and Mycobacterium tuberculosis-induced pro-inflammatory signals. J. Biol. Chem.2005; 48:39914-24.
Jeru I, Duquesnoy P, Fernandes-Alnemri T, et al. Mutations in NALP12 cause hereditary periodic fever syndromes. Proc. Natl. Acad. Sci. USA2008; 105:1614-9.
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