|Application ||WB, ICC, E|
|Other Accession||AAH50556, 29792012|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||72965 Da|
|Application Notes||TCF12 antibody can be used for detection of TCF12 by Western blot at 0.5 - 1 µg/mL. Antibody can also be used for immunocytochemistry starting at 10 µg/mL.|
|Reconstitution & Storage||TCF12 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||TCF12 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||BHLHB20, HEB, HTF4|
|Function||Transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3').|
|Tissue Location||Expressed in several tissues and cell types including skeletal muscle, thymus, and a B-cell line|
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Provided below are standard protocols that you may find useful for product applications.
TCF12 Antibody: TCF12, also known as HTF4, is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. TCF12 is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. TCF12, in combination with E2A, is required to block thymocyte proliferation prior to pre-TCR expression and is critical for proper T cell differentiation. Recent reports have shown that TCF12 is also a critical factor required for the development of invariant natural killer T cells.
Zhang Y and Bina M. The nucelotide sequence of the human transcription factor HTF4a cDNA. DNA Seq.1992; 2:397-403.
Murre C, McCaw PS, Vaesin H, et al. Interactions between heterologous helix-loop-helix proteins generate complexes that bind specifically to a common DNA sequence. Cell1989; 58:537-44.
Wojciechowski J, Lai A, Kondo M, et al. E2A and HEB are required to block thymocyte proliferation prior to pre-TCR expression. J. Immunol.2007; 178:5717-26.
D’Cruz LM, Knell J, Fujimoto JK, et al. An essential role for the transcription factor HEB in thymocyte survival, Tcra rearrangement and the development of natural killer T cells. Nat. Immunol.2010; 11:240-9.
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