|Application ||WB, IF, ICC, E|
|Other Accession||NP_878252, 121949815|
|Calculated MW||16680 Da|
|Application Notes||PLAC4 antibody can be used for detection of PLAC4 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunocytochemistry starting at 10 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||PLAC4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||PLAC4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Tissue Location||Expressed in placental syncytiotrophoblast and choriocarcinoma cells.|
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Provided below are standard protocols that you may find useful for product applications.
PLAC4 Antibody: PLAC4 (placenta-specific protein 4), was initially discovered as a gene whose mRNA was expressed in placental syncytiotrophoblast and choriocarcinoma cells. While the function of PLAC4 is currently unclear, its gene is known to be located on chromosome 21q22.3. Due to the PLAC4 gene location on chromosome 21 and the release of its mRNA transcripts into maternal bloodstream from the placenta, it has been proposed to be a useful marker noninvasive prenatal chromosome 21 aneuploidy detection, although other reports suggests that PLAC4 mRNA levels are not altered in the maternal circulation of pregnancies with trisomy 21.
Kido S, Sakuragi N, Bronner MP, et al. D21S418E identifies a cAMP-regulated gene located on chromosome 21q22.3 that is expressed in placental syncytiotrophoblast and choriocarcinoma. Genomics1993; 17:256-9.
Lo YMD, Tsui NBY, Chiu RWK, et al. Plasma placental RNA allelic ratio permits noninvasive prenatal chromosomal aneuploidy detection. Nat. Med.2007; 13:218-223.
Tsui NB, Akolekar R, Chiu RW, et al. Synergy of total PLAC4 RNA concentration and measurement of the RNA single-nucleotide polymorphism allelic ratio for the noninvasive prenatal detection of trisomy 21. Clin. Chem.2010; 56:73-81.
Banzola I, Rusterholz C, Zannoni L, et al. PLAC4 and beta-HCG mRNA levels are not altered in the maternal circulation of pregnancies with trisomy 21. Prenat. Diagn.2008; 28:1262-7.
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