|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_055252, 21361423|
|Calculated MW||Predicted: 13, 36 kDa |
Observed: 38 kDa
|Application Notes||ZIP1 antibody can be used for detection of ZIP1 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||SLC39A1; At least two isoforms of ZIP1 are known to exist; this antibody will detect both isoforms. ZIP1 antibody is predicted not to cross-react with other ZIP family proteins.|
|Reconstitution & Storage||ZIP1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||ZIP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||IRT1, ZIP1, ZIRTL|
|Function||Mediates zinc uptake. May function as a major endogenous zinc uptake transporter in many cells of the body. Responsible for the rapid uptake and accumulation of physiologically effective zinc in prostate cells.|
|Cellular Location||Cell membrane; Multi-pass membrane protein Endoplasmic reticulum membrane; Multi-pass membrane protein Note=Shows a vesicular localization corresponding partially to the endoplasmic reticulum in several epithelial cell lines|
|Tissue Location||Ubiquitous. Expressed in most adult and fetal tissues including the epidermis.|
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Provided below are standard protocols that you may find useful for product applications.
ZIP1 Antibody: ZIP1, also known as ZIRTL (zinc-iron regulated transporter-like), is the first mammalian member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. ZIP1 expression is markedly downregulated in a number of cancerous tissues and is thought to function as a tumor suppressor gene in prostate cancer. More recent studies have shown that overexpression of ZIP1 and concomitant increased levels of intracellular zinc in PC-3 cells cause a significant inhibition of NF-κB, leading to down-regulation of the antiapoptotic protein Bcl-2, Bcl-XL, and the apoptosis inhibitor XIAP, reducing the malignant potential of prostate cancer cells in vitro and in vivo.
Lioumi M, Ferguson CA, Sharpe PT, et al. Isolation and characterization of human and mouse ZIRTL, a member of the IRT1 family of transporters, mapping within the epidermal differentiation complex. Genomics 1999; 62:272-80.
Eide DJ. The SLC39 family of metal ion transporters. Pflugers Arch. 2004; 447:796-800.
Taylor KM and Nicohlson RI. The LZT proteins; the LIV-1 subfamily of zinc transporters. Biochim. Biophys. Acta. 2003; 1611:16-30.
Costello LC and Franklin RB. The clinical relevance of the metabolism of prostate cancer; zinc and tumor suppression: connecting the dots. Mol. Cancer. 2006; 5:17.
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