|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_071437, 205830403|
|Calculated MW||49631 Da|
|Application Notes||ZIP8 antibody can be used for detection of ZIP8 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||Slc39a8; ZIP8 antibody is predicted to not cross-react with other ZIP family members.|
|Reconstitution & Storage||ZIP8 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||ZIP8 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as a manganese and zinc influx transporter (PubMed:12504855, PubMed:26637978). Plays a role in manganese reabsorption in the proximal tubule of the kidney and in manganese uptake into the brain (PubMed:26637978).|
|Cellular Location||Membrane; Multi-pass membrane protein. Note=Associated with the lysosomal/endosomal compartment following transfection|
|Tissue Location||Expressed in thymus, placenta, lung, liver, pancreas and, to a lower extent, in spleen, testis, ovary, small intestine, colon, leukocyte, heart. Highest expression is observed in pancreas.|
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Provided below are standard protocols that you may find useful for product applications.
ZIP8 Antibody: The zinc transporter ZIP8, also known as SLC39A9, is a member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. The zinc transporter family is divided into four subfamilies (I, II, LIV-1 and gufA). ZIP8 is glycosylated and located at the plasma membrane and mitochondria. It has been identified as the transporter responsible for transport of the toxic Cadmium cation. ZIP8 has also been suggested to play a role in the regulation of interferon-gamma expression in activated human T cells.
Dufner-Beattie J, Langmade SJ, Wang F, et al. Structure, function, and regulation of a subfamily of mouse zinc transporter genes. J. Biol. Chem.2003; 278:50142-50.
Eide DJ. The SLC39 family of metal ion transporters. Pflugers Arch.2004; 447:796-800.
Taylor KM and Nicohlson RI. The LZT proteins; the LIV-1 subfamily of zinc transporters. Biochim. Biophys. Acta.2003; 1611:16-30.
Dalton TP, He L, Wang B, et al. Identification of mouse SLC39A8 as the transporter responsible for cadmium-induced toxicity in the testis. Proc. Natl. Acad. Sci. USA2005; 102:3401-6.
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