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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   KLOTHO Antibody   

KLOTHO Antibody

  • WB - KLOTHO Antibody ASC11255
    Western blot analysis of KLOTHO in HepG2 cell lysate with KLOTHO antibody at (A) 1 and (B) 2 µg/mL.
  • IHC - KLOTHO Antibody ASC11255
    Immunohistochemistry of KLOTHO in mouse heart tissue with KLOTHO antibody at 2.5 µg/mL.
  • IF - KLOTHO Antibody ASC11255
    Immunofluorescence of KLOTHO in mouse heart tissue with KLOTHO antibody at 20 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UEF7
Other Accession NP_004786, 24497614
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 111 kDa

Observed: 106 kDa
Application Notes KLOTHO antibody can be used for detection of KLOTHO by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 9365
Target/Specificity KL; Three isoforms of KLOTHO are known to exist.
Reconstitution & Storage KLOTHO antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsKLOTHO Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KL
Function May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity).
Cellular Location Isoform 1: Cell membrane; Single-pass type I membrane protein. Note=Isoform 1 shedding leads to a soluble peptide Klotho peptide: Secreted.
Tissue Location Present in cortical renal tubules (at protein level). Soluble peptide is present in serum and cerebrospinal fluid. Expressed in kidney, placenta, small intestine and prostate. Down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chronic renal failure kidney
Research Areas
Citations (0)

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KLOTHO Antibody: KLOTHO is the systemic anti-aging hormone within the glycosidase1 superfamily. It encodes a type I membrane protein that is abundant in the kidney and brain. In mice, a deficiency in KLOTHO expression leads to various systemic phenotypes resembling human aging such as arteriosclerosis, osteoporosis, and skin atrophy together with growth retardation, short life-span and infertility. Transgenic mice overexpressing KLOTHO have an extended life span by inhibiting insulin/IGF1 signaling. KLOTHO is involved in the regulation of calcium/phosphorus homeostasis by inhibiting the synthesis of active vitamin D and identified as a potential tumor suppressor.


Kuro-o M, Matsumura Y, and Aizawa H. Mutation of the mouse klotho gene leads to a syndrome resembling ageing. Nature1997; 390:45-51.
Kurosu H, Yamamoto M, Clark JD, et al. Suppression of aging in mice by the hormone Klotho. Science2005; 309:1829-33.
Liu H, Fergusson MM, Castilho RM, et al. Augmented Wnt signaling in a mammalian model of accelerated aging. Science 2007; 317:803-6.
Wolf I, Levanon-Cohen S, Bose S, et al. Klotho: a tumor suppressor and a modulator of the IGF-1 and FGF pathways in human breast cancer. Oncogene2008; 27:7094-105.

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$ 310.00
Cat# ASC11255
(40 western blots)
Availability: 5-7days
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