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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   REQUIEM Antibody   

REQUIEM Antibody

  • WB - REQUIEM Antibody ASC11256
    Western blot analysis of Requiem in mouse kidney tissue lysate with Requiem antibody at 1 µg/mL.
  • IHC - REQUIEM Antibody ASC11256
    Immunohistochemistry of REQUIEM in human kidney tissue with REQUIEM antibody at 5 µg/mL.
  • IF - REQUIEM Antibody ASC11256
    Immunofluorescence of REQUIEM in human kidney tissue with REQUIEM antibody at 20 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q92785
Other Accession AAB81203, 5454004
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 44155 Da
Application Notes REQUIEM antibody can be used for detection of REQUIEM by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 5977
Target/Specificity DPF2; Requiem antibody is predicted to not cross-react with other DPF protein family members.
Reconstitution & Storage REQUIEM antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsREQUIEM Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DPF2
Synonyms BAF45D, REQ, UBID4
Function May be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells.
Cellular Location Nucleus. Cytoplasm. Note=30% nuclear. 70% cytoplasmic
Tissue Location Ubiquitous.
Research Areas
Citations (0)

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REQUIEM Antibody: Requiem is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It is expressed in numerous tissues in both cytosolic and nuclear regions and likely serves a regulatory role in rapid hematopoietic cell growth and turnover. Requiem has been found to act as an adaptor protein that links the NF-κB and SWI/SNF chromatin remodeling factor and is considered a candidate gene for multiple endocrine neoplasia type I, an inherited cancer syndrome involving multiple parathyroid, enteropancreatic, and pituitary tumors.


Gabig TG, Mantel PL, Rosli R, et al. Requiem: a novel zinc finger gene essential for apoptosis in myeloid cells. J. Biol. Chem.1994; 269:29515-9.
Gabig TG, Crean CD, Klenk A, et al. Expression and chromosomal localization of the Requiem gene. Mamm. Genome1998; 9:660-5.
Tando T, Ishizaka A, Watanable H, et al. Requiem protein links RelB/p52 and the Brm-type SWI/SNF complex in a noncanonical NF-kappaB pathway. J. Biol. Chem.2010; 285:21951-60.

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$ 310.00
Cat# ASC11256
(40 western blots)
Availability: 5-7days
Bulk Size
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