|Application ||WB, IHC-P, IF, E|
|Other Accession||AAB81203, 5454004|
|Calculated MW||44155 Da|
|Application Notes||REQUIEM antibody can be used for detection of REQUIEM by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||DPF2; Requiem antibody is predicted to not cross-react with other DPF protein family members.|
|Reconstitution & Storage||REQUIEM antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||REQUIEM Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||BAF45D, REQ, UBID4|
|Function||May be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells.|
|Cellular Location||Nucleus. Cytoplasm. Note=30% nuclear. 70% cytoplasmic|
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REQUIEM Antibody: Requiem is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It is expressed in numerous tissues in both cytosolic and nuclear regions and likely serves a regulatory role in rapid hematopoietic cell growth and turnover. Requiem has been found to act as an adaptor protein that links the NF-κB and SWI/SNF chromatin remodeling factor and is considered a candidate gene for multiple endocrine neoplasia type I, an inherited cancer syndrome involving multiple parathyroid, enteropancreatic, and pituitary tumors.
Gabig TG, Mantel PL, Rosli R, et al. Requiem: a novel zinc finger gene essential for apoptosis in myeloid cells. J. Biol. Chem.1994; 269:29515-9.
Gabig TG, Crean CD, Klenk A, et al. Expression and chromosomal localization of the Requiem gene. Mamm. Genome1998; 9:660-5.
Tando T, Ishizaka A, Watanable H, et al. Requiem protein links RelB/p52 and the Brm-type SWI/SNF complex in a noncanonical NF-kappaB pathway. J. Biol. Chem.2010; 285:21951-60.
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