|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_003217, 119629977|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 10 kDa |
Observed: 11 kDa
|Application Notes||TFF3 antibody can be used for detection of TFF3 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||TFF3; TFF3 antibody is predicted to not cross-react with other TFF family members.|
|Reconstitution & Storage||TFF3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||TFF3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).|
|Cellular Location||Secreted, extracellular space, extracellular matrix. Cytoplasm|
|Tissue Location||Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus. Also expressed in the hypothalamus where it is detected in paraventricular, periventricular and supraoptic nuclei (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
TFF3 Antibody: Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. Trefoil peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain and clustered in human chromosome 21q22.3. Trefoil Factor 3 (TFF3) is one of three trefoil peptides secreted by epithelial cells that line mucus membranes in the large and small intestines and lower respiratory tract. TFF3 is mainly active as a disulfide-linked homodimer and may have a role in promoting cell migration in healing processes. It is involved in the maintenance and repair of the intestinal mucosa.
Podolsky DK, Lynch-Devaney K, Stow JL, et al. Identification of human intestinal trefoil factor. Goblet cell-specific expression of a peptide targeted for apical secretion. J. Biol. Chem. 1993; 268:6694-702.
Gott P, Beck S, Machado JC, et al. Human trefoil peptides: genomic structure in 21q22.3 and coordinated expression. Eur. J. Hum. Genet. 1996; 4:308-15.
Hernandez C, Santamatilde E, McCreath KJ, et al. Induction of trefoil factor TFF1, TFF2 and TFF3 by hypoxia is mediated by hypoxia inducible factor-1: implications for gastric mucosal healing. Br. J. Pharmacol. 2009; 156:262-72.
Thim L. Trefoil peptides: from structure to function. Cell Mol. Life Sci. 1997; 53:888-903.
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