|Application ||WB, IHC-P, IF, E|
|Other Accession||AAH84568, 7705375|
|Calculated MW||61439 Da|
|Application Notes||ZBTB7A antibody can be used for detection of ZBTB7A by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||ZBTB7A; At least two isoforms of ZBTB7A are known to exist; this antibody will recognize both isoforms. This antibody is predicted to not cross-react with other ZBTB protein family members.|
|Reconstitution & Storage||ZBTB7A antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||ZBTB7A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||FBI1, LRF, ZBTB7, ZNF857A|
|Function||Plays a key role in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals (By similarity). Specifically represses the transcription of the CDKN2A gene. Efficiently abrogates E2F1- dependent CDKN2A transactivation/de-repression. Binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3' (By similarity).|
|Tissue Location||Widely expressed. In normal thymus, expressed in medullary epithelial cells and Hassle's corpuscles (at protein level). In tonsil, expressed in squamous epithelium and germinal center lymphocytes (at protein level). Up-regulated in a subset of lymphomas, as well as in a subset of breast, lung, colon, prostate and bladder carcinomas (at protein level)|
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Provided below are standard protocols that you may find useful for product applications.
ZBTB7A Antibody: The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. ZBTB7A, also known as LRF, was initially identified as a potential target of the LAZ-3/BCL-6 oncogene. Later reports demonstrated that it could bind to the wild-type IST (inducer of short transcripts) elements of HIV-1 long terminal repeats (LTRs) and stimulate Tat activity on the HIV-1 LTR. More recently it ZBTB7A has been recognized as a proto-oncogene whose overexpression contributes to malignancy in breast cancer and functions as a co-repressor of the androgen receptor in prostate cancer cells.
Filion GJP, Zhenilo S, Salozhin S, et al. A family of zinc finger proteins that bind methylated DNA and repress transcription. Mol. Cell. Biol.2006; 26:169-81.
Davies JM, Hawe N, Kabarowski J, et al. Novel BTB/POZ domain zinc-finger protein, LRF, is a potential target of the LAZ-3/BCL-6 oncogene. Oncogene1999; 18:365-75.
Pendergrast PS, Wang C, Hernandez N, et al. FBI-1 can stimulate HIV-1 Tat activity and is targeted to a novel subnuclear domain that includes the Tat-P-TEFb-containing nuclear speckles. Mol. Cell. Biol.2002; 13:915-29.
Qu H, Qu D, Chen F, et al. ZBTB7 overexpression contributes to malignancy in breast cancer. Cancer Invest.2010; 28:672-8.
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