|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_821079, 109809759|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||65896 Da|
|Application Notes||LRRTM3 antibody can be used for detection of LRRTM3 by Western blot at 0.5 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||LRRTM3; At least two isoforms of LRRTM3 are known to exist; this antibody will only detect the larger isoform.|
|Reconstitution & Storage||LRRTM3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||LRRTM3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Exhibits a limited synaptogenic activity in vitro, restricted to excitatory presynaptic differentiation (By similarity). May play a role in the development and maintenance of the vertebrate nervous system.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein. Cell junction, synapse, postsynaptic cell membrane; Single-pass type I membrane protein|
|Tissue Location||Expressed in neuronal tissues.|
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Provided below are standard protocols that you may find useful for product applications.
LRRTM3 Antibody: The Leucine-rich repeat transmembrane neuronal proteins (LRRTMs) are differentially expressed in the nervous system and were recently found to instruct presynaptic and mediate postsynaptic glutamatergic differentiation, with LRRTM1 and LRRTM2 most potent at inducing presynaptic differentiation. Recent evidence suggests that LRRTM3 promotes the processing of amyloid-precursor protein (APP) by the beta-secretase BACE, leading to the proteolytic production of the Abeta42 peptide that is the main component of amyloid plaques. Furthermore, LRRTM3 maps to a region of chromosome 10 linked to both late-onset Alzheimer's disease (AD) and elevated plasma Abeta42 levels, suggesting that LRRTM3 is a functional and positional candidate gene for AD.
Lauren J, Airaksinen MS, Saarma M, et al. A novel gene family encoding leucine-rich repeat transmembrane protein differentially expressed in the nervous system. Genomics 2003; 81:411-21.
Linhoff MW, Lauren J, Cassidy RM, et al. An unbiased expression screen for synaptogenic proteins identifies the LRRTM protein family as synaptic organizers. Neuron 2009; 61:734-49.
Siddiqui TJ, Pancaroglu R, Kang Y, et al. LRRTMs and neuroligins bind neurexins with a differential code to cooperate in glutamate synapse development. J. Neurosci. 2010; 30:7495-506.
Majercak J, Ray WJ, Espeseth A, et al. LRRTM3 promotes processing of amyloid-precursor protein by BACE1 and is a positional candidate gene for late-onset Alzheimer’s disease. Proc. Natl. Acad. Sci. USA 2006; 47:17967-72.
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