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N4BP1 Antibody

  • WB - N4BP1 Antibody ASC11278
    Western blot analysis of N4BP1 in HeLa cell lysate with N4BP1 antibody at 0.5 µg/mL in (A) the absence and (B) the presence of blocking peptide.
  • ICC - N4BP1 Antibody ASC11278
    Immunocytochemistry of N4BP1 in HeLa cells with N4BP1 antibody at 10 µg/mL.
  • IF - N4BP1 Antibody ASC11278
    Immunofluorescence of N4BP1 in HeLa cells with N4BP1 antibody at 20 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O75113
Other Accession NP_694574, 48928019
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 100379 Da
Application Notes N4BP1 antibody can be used for detection of N4BP1 by Western blot at 0.5 µg/mL. Antibody can also be used for immunocytochemistry starting at 10 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 9683
Target/Specificity N4BP1; At least three isoforms of N4BP1 are known to exist; this antibody will only detect the longest isoform.
Reconstitution & Storage N4BP1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsN4BP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name N4BP1
Synonyms KIAA0615
Function Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates (By similarity).
Cellular Location Nucleus, nucleolus. Nucleus, PML body. Note=Primarily localizes to the nucleolus Also localizes to the PML nuclear bodies, when desumoylated (By similarity).
Tissue Location Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary
Citations (0)

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N4BP1 Antibody: Nedd4-binding partner-1 (N4BP1) is a developmentally expressed protein interactor and monoubiquitylation substrate of Nedd4, a HECT domain-containing ubiquitin ligase that mediates ubiquitylation and proteasome degradation of target proteins and may have a fundamental role to play in embryonic processes. N4BP1 can undergo Nedd4-mediated polyubiquitylation and proteosomal degradation. N4BP1 also functions as a negative regulator of AIF4 (also known as ITCH or Atrophin-1), an E3-ubiquitin ligase, by interacting with the second WW domain of AIF4 and impairing ubiquitination of substrates.


Murillas R, Simms KS, Hatakeyama S, et al. Identification of developmentally expressed proteins that functionally interact with Nedd4 ubiquitin ligase. J. Biol. Chem. 2002; 277:2897-907.
Sharma P, Murillas R, Zhang H, et al. N4BP1 is a newly identified nucleolar protein that undergoes SUMO-regulated polyubiquitylation and proteasomal turnover at promyelocytic leukemia nuclear bodies. J. Cell Sci. 2010; 123:1227-34.
Oberst A, Malatesta M, Aqeilan RI, et al. The Nedd4-binding partner 1 (N4BP1) protein is an inhibitor of the E3 ligase Itch. Proc. Natl. Acad. Sci. USA 2007; 104:11280-5.

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$ 330.00
Cat# ASC11278
Availability: 5-7days
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