|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_036411, 6912440|
|Calculated MW||32933 Da|
|Application Notes||ZNF346 antibody can be used for detection of ZNF346 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||ZNF346; ZNF346 antibody is predicted to not cross-react with other ZNF family members. Three isoforms of ZNF346 are known to exist; this antibody will recognize the two longest isoforms|
|Reconstitution & Storage||ZNF346 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||ZNF346 (CT) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds with low affinity to dsDNA and ssRNA, and with high affinity to dsRNA, with no detectable sequence specificity.|
|Cellular Location||Nucleus, nucleolus. Cytoplasm. Note=Nuclear at steady state, primarily in the nucleolus. Shuttles between the nucleus and cytoplasm when associated with XPO5|
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Provided below are standard protocols that you may find useful for product applications.
ZNF346 (CT) Antibody: ZNF346, also known as JAZ (just another zinc-finger protein), is a nucleolar, zinc finger protein which preferentially binds to double-stranded (ds) RNA or RNA/DNA hybrids with high affinity via C2H2 zinc fingers. ZNF346 contains four CH-type zinc finger motifs that are connected by long (28-38) amino acid linker sequences. ZNF346 is expressed in all tissues tested and localizes to the nucleus, primarily the nucleolus. ZNF346 is exported by exportin-5 but translocates back into nuclei by a facilitated diffusion mechanism. ZNF346 interacts with ILF3 in an RNA-independent manner and may be involved in cell growth and survival.
Rosenfeld R and Margalit H. Zinc fingers: conserved properties that can distinguish between spurious and actual DNA-binding motifs. J. Biomol. Struct. Dyn. 1993; 11:557-70.
Simpson JC, Wellenreuther R, Poustka A, et al. Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing. EMBO Rep. 2000; 1:287-92.
Yang M, May WS, and Ito T. JAZ requires the double-stranded RNA-binding zinc-finger motifs for nuclear localization. J. Biol. Chem. 1999; 274: 27399-406.
Chen T, Brownawell AM, and Macara IG. Nucleocytoplasmic shuttling of JAZ, a new cargo protein for Exportin 5. Mol. Cell. Biol. 2004; 24:6608-19.
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