|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_037399, 20336214|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 36, 55, 62, 66 kDa |
Observed: 55, 62 kDa
|Application Notes||GLS2 antibody can be used for detection of GLS2 by Western blot at 0.5 - 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||GLS2; Multiple isoforms of GLS2 are known to exist.|
|Reconstitution & Storage||GLS2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||GLS2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.|
|Tissue Location||Highly expressed in liver. Expressed in brain and pancreas. Not observed in heart, placenta, lung, skeletal muscle and kidney. Expression is significantly reduced in hepatocellular carcinomas.|
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Provided below are standard protocols that you may find useful for product applications.
GLS2 Antibody: Phosphate-activated glutaminase, also known as Glutaminase 2 (GLS2), was initially isolated from rat liver, although it has been shown to be expressed in other tissues. Like the functionally similar, larger kidney glutaminase, GLS2 catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Expression of GLS2 is increased by p53 under both stressed and nonstressed conditions, resulting in increased levels of glutamate and alpha-ketoglutarate, which in turn results in enhanced mitochondrial respiration and ATP generation. GLS2 also regulates antioxidant defense function in cells by increasing reduced glutathione levels and decreasing ROS-levels, suggesting that GLS2 acts as a mediator of p53's role in antioxidant defense in addition to its role in energy metabolism.
Chung-Bok MI, Vincent N, Jhala U, et al. Rat hepatic glutaminase: identification of the full coding sequence and characterization of a functional promoter. Biochem. J. 1997; 324:193-200.
Gomez-Fabre PM, Aledo JC, del Castillo-Olivares A, et al. Molecular cloning, sequencing and expression studies of the human breast cancer cell glutaminase. Biochem. J. 2000; 345:365-75.
Hu W, Zhang C, Wu R, et al. Glutaminase 2, a novel p53 target gene regulating energy metabolism and antioxidant function. Proc. Natl. Acad. Sci. USA 2010; 107:7455-60.
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