|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_001018110, 70166414|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||54206 Da|
|Application Notes||MYOZAP antibody can be used for detection of MYOZAP by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||GCOM1; Multiple isoforms of MYOZAP are known to exist.|
|Reconstitution & Storage||MYOZAP antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||MYOZAP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role in cellular signaling via Rho-related GTP- binding proteins and subsequent activation of transcription factor SRF (By similarity). Targets TJP1 to cell junctions. In cortical neurons, may play a role in glutaminergic signal transduction through interaction with the NMDA receptor subunit GRIN1 (By similarity).|
|Cellular Location||Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, myofibril, sarcomere, I band. Cytoplasm, myofibril, sarcomere, Z line. Cell junction. Note=Detected predominantly at the intercalated disk in cardiomyocytes, and at low levels on sarcomeric Z disks. Colocalizes with F-actin. Colocalizes with cortical actin (By similarity).|
|Tissue Location||Detected in heart, liver, skeletal muscle, placenta, small intestine, lung, prostate and testis|
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Provided below are standard protocols that you may find useful for product applications.
MYOZAP Antibody: MYOZAP, also known as GRINL1A, is a 54 kDa highly conserved cardiac protein. It is strongly expressed in the heart and lung and is a novel component of intercalated disc. MYOZAP interacts with myosin phosphatase-RhoA interacting protein (MRIP) and acts as an activator of Rho-dependent SRF signaling. Knockdown study in zebrafish results in cardiomyopathy with severe dysfunction. The MYOZAP gene is part of a complex transcript unit that includes the gene for glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A (GRINL1A). Transcription of this gene occurs at an upstream promoter, with two different groups of alternatively spliced variants: Gup for GRINL1A upstream transcripts and Gcom for GRINL1A combined transcripts.
Van Tintelen JP, Hofstra RM, Wiesfeld AC, et al. Molecular genetics of arrhythmogenic right ventricular cardiomyopathy: emerging horizon? Curr. Opin. Cardiol. 2007; 22:185-92.
Seeger TS, Frank D, Rohr C, et al. Myozap, a novel intercalated disc protein, activates serum response factor-dependent signaling and is required to maintain cardiac function in vivo. Circ. Res. 2010; 106:880-90.
Roginski RS, Mohan Raj BK, Birditt B, et al. The human GRINL1A gene defines a complex transcription unit, an unusual form of gene organization in eukaryotes. Genomics 2004; 84:265-76.
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