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ZFP219 Antibody

  • WB - ZFP219 Antibody ASC11298
    Western blot analysis of ZFP219 in mouse brain tissue lysate with ZFP219 antibody at (A) 1 and (B) 2 µg/mL.
  • IHC - ZFP219 Antibody ASC11298
    Immunohistochemistry of ZFP219 in mouse brain tissue with ZFP219 antibody at 5 µg/mL.
  • IF - ZFP219 Antibody ASC11298
    Immunofluorescence of ZFP219 in mouse brain tissue with ZFP219 antibody at 20 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9P2Y4
Other Accession NP_001095142, 156415996
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 76877 Da
Application Notes ZFP219 antibody can be used for detection of ZFP219 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 51222
Target/Specificity ZNF219; At least two isoforms of ZFP219 are known to exist; this antibody will recognize both.
Reconstitution & Storage ZFP219 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsZFP219 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ZNF219
Function May be involved in transcriptional regulation.
Cellular Location Nucleus.
Tissue Location Ubiquitous.
Research Areas
Citations (0)

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ZFP219 Antibody: ZFP219 is a developmentally regulated member of the Kruppel-like zinc finger gene family that is thought to function as a transcriptional repressor. Yeast two-hybrid screening showed association with Sox9, a transcription factor that is essential for chondrogenesis. ZFP219 is specifically expressed in the developing limb buds and colocalizes with Sox9 in the nucleus. Knockdown of ZFP219 expression decreased Sox9-induced mRNA expression, and a dominant-negative mutant of ZFP219 inhibited Bmp2-induced chondrocyte differentiation, suggesting that ZFP219 plays an important role as a transcriptional partner of Sox9 in the regulation of chondrocyte differentiation.


Sakai T, Toyoda A, Hashimoto K, et al. Isolation and characterization of a novel zinc finger gene, ZNF219, and mapping to the human chromosome 14q11 region. DNA Res. 2000; 7:137-41.
Sakai T, Hino K, Wada S, et al. Identification of the DNA binding specificity of the human ZNF219 protein and its function as a transcriptional repressor. DNA Res. 2003; 10:155-65.
Takigawa Y, Hata K, Muramatsu S, et al. The transcription factor Znf219 regulates chondrocyte differentiation by assembling a transcription factory with Sox9. J. Cell Sci. 2010; 123:3780-8.

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$ 330.00
Cat# ASC11298
Availability: 5-7days
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