|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_001095142, 156415996|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||76877 Da|
|Application Notes||ZFP219 antibody can be used for detection of ZFP219 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||ZNF219; At least two isoforms of ZFP219 are known to exist; this antibody will recognize both.|
|Reconstitution & Storage||ZFP219 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||ZFP219 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May be involved in transcriptional regulation.|
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Provided below are standard protocols that you may find useful for product applications.
ZFP219 Antibody: ZFP219 is a developmentally regulated member of the Kruppel-like zinc finger gene family that is thought to function as a transcriptional repressor. Yeast two-hybrid screening showed association with Sox9, a transcription factor that is essential for chondrogenesis. ZFP219 is specifically expressed in the developing limb buds and colocalizes with Sox9 in the nucleus. Knockdown of ZFP219 expression decreased Sox9-induced mRNA expression, and a dominant-negative mutant of ZFP219 inhibited Bmp2-induced chondrocyte differentiation, suggesting that ZFP219 plays an important role as a transcriptional partner of Sox9 in the regulation of chondrocyte differentiation.
Sakai T, Toyoda A, Hashimoto K, et al. Isolation and characterization of a novel zinc finger gene, ZNF219, and mapping to the human chromosome 14q11 region. DNA Res. 2000; 7:137-41.
Sakai T, Hino K, Wada S, et al. Identification of the DNA binding specificity of the human ZNF219 protein and its function as a transcriptional repressor. DNA Res. 2003; 10:155-65.
Takigawa Y, Hata K, Muramatsu S, et al. The transcription factor Znf219 regulates chondrocyte differentiation by assembling a transcription factory with Sox9. J. Cell Sci. 2010; 123:3780-8.
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