|Application ||WB, ICC, E|
|Other Accession||NP_001387, 18765758|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||85584 Da|
|Application Notes||DYRK1A antibody can be used for detection of DYRK1A by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunocytochemistry starting at 10 µg/mL.|
|Target/Specificity||DYRK1A; Multiple isoforms of DYRK1A are known to exist. DYRK1A antibody will not cross-react with other DYRK family members|
|Reconstitution & Storage||DYRK1A antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||DYRK1A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||DYRK, MNB, MNBH|
|Function||May play a role in a signaling pathway regulating nuclear functions of cell proliferation. Modulates alternative splicing by phosphorylating the splice factor SRSF6 (By similarity). Phosphorylates serine, threonine and tyrosine residues in its sequence and in exogenous substrates such as CRY2, FOXO1, SRSF6 and SIRT1. Exhibits a substrate preference for proline at position P+1 and arginine at position P-3.|
|Cellular Location||Nucleus speckle.|
|Tissue Location||Ubiquitous. Highest levels in skeletal muscle, testis, fetal lung and fetal kidney.|
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Provided below are standard protocols that you may find useful for product applications.
DYRK1A Antibody: DYRK1A is a member of the dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family and is thought to be involved in neuronal differentiation, neurodegenerative diseases, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. DYRK1A phosphorylates several transcription factors such as p53, leading to inhibition of embryonic neuronal cell proliferation. DYRK1A associates with multiple proteins, including SPRED1 and SPRED2; this association appears to inhibit the phosphorylation activity of DYRK1A. Recent evidence also suggests that DYRK1A may be involved in the replication of HIV-1.
Park J, Oh Y, and Chung KC. Two key genes closely implicated with the neuropathological characteristics in Down Syndrome: DYRK1A and RCAN1. BMB Reports 2009; 42:6-15.
Park J, Oh Y, You L, et al. Dyrk1A phosphorylates p53 and inhibits proliferation of embryonic neuronal cells. J. Biol. Chem. 2010; 285:31895-906
Li D, Jackson RA, Yusoff P, et al. Direct association of Sprouty-related protein with and EVH1 domain (SPRED) 1 or SPRED2 with DYRK1A modifies substrate/kinase interactions. J. Biol. Chem. 2010; 285:35374-85.
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