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ApoA4 Antibody

     
  • WB - ApoA4 Antibody ASC11312
    Western blot analysis of ApoA4 in chicken small intestine tissue lysate with ApoA4 antibody at 1 µg/mL
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P06727
Other Accession NP_990269, 337
Reactivity Human, Chicken
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 45372 Da
Application Notes ApoA4 antibody can be used for detection of ApoA4 by Western blot at 1 μg/mL.
Additional Information
Gene ID 337
Target/Specificity ApoA4 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of chicken ApoA4.

The immunogen is located within the last 50 amino acids of ApoA4.
Reconstitution & Storage ApoA4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsApoA4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name APOA4
Function May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.
Cellular Location Secreted.
Tissue Location Synthesized primarily in the intestine and secreted in plasma
Research Areas
Citations (0)
citation

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Background

ApoA4 Antibody: Apolipoprotein A4 (also known as ApoA-IV) is a plasma protein that is O-linked glycoprotein after proteolytic processing. ApoA4 is a major constituent of HDL and it also can be found on the surface of newly synthesized chylomicrons. The physiological function of ApoA4 is not completely identified so far, but several evidences from vitro studies indicate that this apolipoprotein involves in several steps of the reverse cholesterol transport pathway, which transports cholesterol from peripheral cells back to the liver.

References

Karathanasis SK, Oettgen P, Haddad IA, et al. Structure, evolution, and polymorphisms of the human apolipoprotein A4 gene (APOA4). Proc. Natl. Acad. Sci. 1986; 83:8457-61
Kamboh MI, Friedlaender JS, Ahn YI et al. A common deletion polymorphism in the apolipoprotein A4 gene and tis significance in lipid metabolism. Arterioscler. Thromb. 1994; 14:656-62.
Kronenberg F, Stuhlinger M, Trenkwalder E, et al. Low apolipoprotein A-IV plasma concentrations in men with coronary artery disease. J. Am. Coll. Cardiol., 2000; 36:751-7
Von Eckardstein A, Huang Y, Wu S, et al. Lipoproteins containing apolipoprotein A-IV but not apolipoprotein A-I take up and esterify cell-derived cholesterol in plasma. Arterioscler Thromb Vasc Biol. 1995; 15:1755-63.

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$ 379.00
Cat# ASC11312
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