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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   TYW4 Antibody   

TYW4 Antibody

  • WB - TYW4 Antibody ASC11336
    Western blot analysis of TYW4 in rat brain tissue lysate with TYW4 antibody at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.
  • IHC - TYW4 Antibody ASC11336
    Immunohistochemistry of TYW4 in mouse brain tissue with TYW4 antibody at 5 µg/mL.
  • IF - TYW4 Antibody ASC11336
    Immunofluorescence of TYW1 in mouse brain tissue with TYW1 antibody at 20 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O60294
Other Accession NP_055608, 25188199
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 75602 Da
Application Notes TYW4 antibody can be used for detection of TYW4 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 9836
Target/Specificity LCMT2; TYW4 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW4 are known to exist; this antibody will detect only the long isoform.
Reconstitution & Storage TYW4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsTYW4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LCMT2
Synonyms KIAA0547, TYW4
Function Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA (By similarity). May methylate the carboxyl group of leucine residues to form alpha- leucine ester residues.
Research Areas
Citations (0)

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TYW4 Antibody: TYW4 is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW4 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation.


Noma A and Suzuki T. Ribonucleome analysis identified enzyme genes responsible for wybutosine synthesis. Nucleic Acids Symp. Ser. (Oxf) 2006; 65-6.
Noma A, Kirino Y, Ikeuchi Y, et al. Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA. EMBO J. 2006; 25:2142-54.

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$ 310.00
Cat# ASC11336
(40 western blots)
Availability: 5-7days
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