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GEMC1 Antibody

  • WB - GEMC1 Antibody ASC11339
    Western blot analysis of GEMC1 in mouse heart tissue lysate with GEMC1 antibody at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession A6NCL1
Other Accession NP_001140158, 226442751
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 37887 Da
Application Notes GEMC1 antibody can be used for detection of GEMC1 by Western blot at 1 µg/mL.
Additional Information
Gene ID 647309
Target/Specificity GEMC1;
Reconstitution & Storage GEMC1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsGEMC1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms GEMC1
Function Regulator of DNA replication. Promotes initiation of chromosomal DNA replication by mediating TOPBP1- and CDK2- dependent recruitment of CDC45L onto replication origins (By similarity).
Cellular Location Nucleus. Note=Associates with chromatin during pre-replication complex (pre-RC) formation
Citations (0)

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GEMC1 Antibody: The Geminin coiled-coil domain-containing protein 1 (GEMC1) is a highly conserved protein that is required for chromosomal replication. GEMC1 contains a region similar to the coiled-coil domain of geminin, an essential vertebrate replication protein in which the coiled-coil domain is required for the function. GEMC1 binds to the checkpoint and replication factor TopBP1, allowing the binding of GEMC1 to chromatin during pre-replication complex formation. GEMC1 also directly interacts with replication factors Cdc45 and Cdk2-CyclinE and stimulates the initiation of DNA replication. Depletion of GEMC1 by siRNA in embryonic and somatic vertebrate cells prevents DNA replication due to impairment of Cdc45 loading onto chromatin, suggesting GEMC1 promotes initiation of DNA replication by mediating TopBP1 and Cdk2 dependent recruitment of Cdc45 onto replication origins.


Piergiovanni G, Costanzo V. GEMC1 is a novel TopBP1-interacting protein involved in chromosomal DNA replication. Cell Cycle 2010; 9:3662-6.
McGarry TJ and Kirschner MW. Geminin, an inhibitor of DNA replication, is degraded during mitosis. Cell 1998; 93:1043-53.
Rowles A and Blow JJ. Chromatin proteins involved in the initiation of DNA replication. Curr. Opin. Genet. Dev. 1997; 7:152-7.
Balestrini A, Cosentino C, Errico A, et al. GEMC1 is a TopBP1 interacting protein required for chromosomal DNA replication. Nat. Cell Biol. 2010; 12:484-91.

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$ 330.00
Cat# ASC11339
Availability: 5-7days
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