|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_705838, 62241003|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||449211 Da|
|Application Notes||SPRYD2 antibody can be used for detection of SPRYD2 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||CMYA5; SPRYD2 antibody is predicted to not cross-react with other SPRYD protein family members. At least four isoforms of SPRYD2 are known to exist.|
|Reconstitution & Storage||SPRYD2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||SPRYD2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||C5orf10, DTNBP2, SPRYD2, TRIM76|
|Function||May serve as an anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) via binding to PRKAR2A (By similarity). May function as a repressor of calcineurin-mediated transcriptional activity. May attenuate calcineurin ability to induce slow-fiber gene program in muscle and may negatively modulate skeletal muscle regeneration (By similarity).|
|Cellular Location||Cytoplasm. Cytoplasm, perinuclear region. Cytoplasm, myofibril, sarcomere, M line. Note=Found predominantly at the periphery of the nucleus but also throughout the cell Localized in lysosomes (By similarity). In skeletal muscles, localizes along myofiber periphery, at costameres (By similarity) Predominantly flanks Z-disks. Occasionnally present at the M-band level.|
|Tissue Location||Expressed in skeletal muscle; at a strong level and in heart.|
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Provided below are standard protocols that you may find useful for product applications.
SPRYD2 Antibody: SPRYD2, also known as Myospryn, was originally identified as the muscle-specific partner of dysbindin and as a Mef-2 target gene. It is a large scaffolding protein localized to the Z-disc/costamere region of striated muscle. SPRYD2 includes a noncanonical tripartite motif (TRIM-like) that lacks the RING domain but consists of a B-box coiled coil (BBC), fibronectin 3 (FN3) repeats, and SPRY domains. SPRYD2 interacts with desmin and calcineurin, and it has been suggested to play a role in the biogenesis of lysosome and negatively regulates slow-fiber-type transformation and skeletal muscle regeneration. SPRYD2 is dysregulated in Duchenne muscular dystrophy.
Benson MA, Tinsley CL, Blake DJ. Myospryn is a novel binding partner for dysbindin in muscle. J. Biol. Chem. 2004; 279:10450-8.
Sarparanta J. Biology of myospryn: what’s known? J. Muscle Res. Cell Motil. 2008; 29:177-80
Durham JT, Brand OM, Arnold M, et al. Myospryn is a direct transcriptional target for MEF2A that encodes a striated muscle, alpha-actinin-interacting, costamere-localized protein. J. Biol. Chem. 2006; 281:6841-9
Kielbasa OM, Reynolds JG, Wu CL, et al. Myospryn is a calcineurin-interacting protein that negatively modulates slow-fiber-type transformation and skeletal muscle regeneration. FASEB J. 2011 epub.
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