|Application ||WB, IHC-P, E|
|Other Accession||NP_620135, 254039650|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||81523 Da|
|Application Notes||SYTL5 antibody can be used for detection of SYTL5 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL.|
|Target/Specificity||SYTL5; Multiple isoforms of SYTL5 are known to exist. SYTL5 antibody is predicted to not cross-react with other SYTL protein family members.|
|Reconstitution & Storage||SYTL5 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||SYTL5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May act as Rab effector protein and play a role in vesicle trafficking. Binds phospholipids.|
|Cellular Location||Membrane; Peripheral membrane protein|
|Tissue Location||Highly expressed in placenta and liver.|
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Provided below are standard protocols that you may find useful for product applications.
SYTL5 Antibody: SYTL5 belongs to the synaptotagmin-like (Slp) protein family, which contains a unique Slp homolog domain (SHD) at the N-terminus. The SHD functions as a binding site for Rab27A, which plays a role in protein transport. Expression of SYTL5 is restricted to placenta and liver, suggesting that it might be involved in Rab27A-dependent membrane trafficking in specific tissues. Several members of the synaptotagmin family are calcium-sensing vesicle-fusion proteins as mediators of cell migration. SYTL7 & SYTL5 were positive regulators of chemotaxis, whereas SYTL2 was a negative regulator of chemotaxis.
Kuroda TS, Fukuda M, Ariga H, et al. Synaptotagmin-like protein 5: a novel Rab27A effector with C-terminal tandem C2 domains. Biochem. Biophys. Res. Commun. 2002; 293:899-906.
Saxena SK, Horiuchi H, and Fukuda M. Rab27a regulates epithelial sodium channel (ENaC) activity through synaptotagmin-like protein (SLP-5) and Munc13-4 effector mechanism. Biochem. Biophys. Res. Commun. 2006; 344:651-7.
Colvin RA, Means TK, Diefenbach TJ, et al. Synaptotagmin-mediated vesicle fusion regulates cell migration. Nat. Immunol. 2010; 11:495-502
Fukuda M, Mikoshiba K. Synaptotagmin-like protein 1-3: a novel family of C-terminal-type tandem C2 proteins. Biochem. Biophys. Res. Commun. 2001; 281:1226-33.
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