|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_036241, 6912274|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||37619 Da|
|Application Notes||TMEM59L antibody can be used for detection of TMEM59L by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||TMEM59L; Multiple isoforms of TMEM59L are known to exist. TMEM59L antibody is predicted to not cross-react TMEM59.|
|Reconstitution & Storage||TMEM59L antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||TMEM59L Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Modulates the O-glycosylation and complex N- glycosylation steps occurring during the Golgi maturation of APP. Inhibits APP transport to the cell surface and further shedding.|
|Cellular Location||Golgi apparatus membrane; Single-pass type I membrane protein|
|Tissue Location||Expressed preferentially at high level in the brain|
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Provided below are standard protocols that you may find useful for product applications.
TMEM59L Antibody: Processing of the amyloid precursor protein (APP) by two different proteases, called alpha- and beta-secretase, is a central regulatory event in the generation of the amyloid beta peptide (Abeta), which has a key role in Alzheimer disease (AD) pathogenesis. TMEM59L is a type I membrane glycoprotein that is expressed in the brain. A related protein, TMEM59, is a Golgi-localized protein that modulates the O-glycosylation and complex N-glycosylation steps occurring during the Golgi maturation of several proteins such as APP, BACE1, SEAP or PRNP and inhibits APP transport and shedding.
Schöbel S, Neumann S, Seed B, et al. Expression cloning screen for modifiers of amyloid precursor protein shedding. Int. J. Dev. Neurosci. 2006; 24:141-8.
Schöbel S, Neumann S, Hertweck M, et al. A novel sorting nexin modulates endocytic trafficking and alpha-secretase cleavage of the amyloid precursor protein. J. Biol. Chem. 2008; 283:14257-68.
Elson GC, de Coignac AB, Aubry JP, et al. BSMAP, a novel protein expressed specifically in the brain whose gene is localized on chromosome 19p12. Biochem. Biophys. Res. Commun. 1999; 264:55-62.
Ullrich S, Münch A, Neumann S, et al. The novel membrane protein TMEM59 modulates complex glycosylation, cell surface expression, and secretion of the amyloid precursor protein. J. Biol. Chem. 2010; 285:20664-74.
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