|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_078811, 168229161|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||100605 Da|
|Application Notes||Coronin 7 antibody can be used for detection of Coronin 7 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||CORO7; Multiple isoforms of Coronin 7 are known to exist|
|Reconstitution & Storage||Coronin 7 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||Coronin 7 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||F-actin regulator involved in anterograde Golgi to endosome transport: upon ubiquitination via 'Lys-33'-linked ubiquitin chains by the BCR(KLHL20) E3 ubiquitin ligase complex, interacts with EPS15 and localizes to the trans-Golgi network, where it promotes actin polymerization, thereby facilitating post- Golgi trafficking. May play a role in the maintenance of the Golgi apparatus morphology.|
|Cellular Location||Golgi apparatus membrane. Golgi apparatus, trans-Golgi network. Cytoplasmic vesicle. Cytoplasm, cytosol. Note=Predominantly cytosolic. Detected on vesicle-like cytoplasmic structures and on the cis-Golgi. Not associated with actin filaments|
|Tissue Location||Widely expressed. Expressed in the spleen, peripheral leukocytes, testes, brain, thymus and small intestine|
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Provided below are standard protocols that you may find useful for product applications.
Coronin 7 Antibody: Coronins, such as Coronin 7, constitute an evolutionarily conserved family of WD-repeat actin-binding proteins whose function is thought to be regulating the cytoskeleton. Unlike other cytoplasmically localized Coronins, Coronin 7 localizes in part to the Golgi complex and is thought to play a role in Golgi complex morphology and function. Other experiments indicate that Coronin 7 interacts with the anti-proliferative protein TOB1 and enhances its degradation though the SCF ubiquitin ligase (E3) complex.
Rybakin V, Stumpf N, Schulze A, et al. Coronin 7, the mammalian POD-1 homologue, localizes to the Golgi apparatus. FEBS Lett. 2004; 573:161-7.
Crn7 interacts with AP-1 and is required for the maintenance of Golgi morphology and protein export from the Golgi. J. Biol. Chem. 2006; 281:31070-8.
Watanabe M, Suzuki T, Kim M, et al. Coronin7 forms a novel E3 ubiquitin ligase complex to promote the degradation of the anti-proliferative protein Tob. FEBS Lett. 2011; 585:65-70.
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