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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   NETO1 Antibody   

NETO1 Antibody

     
  • WB - NETO1 Antibody ASC11370
    Western blot analysis of NETO1 in human lung tissue lysate with NETO1 antibody at (A) 1 and (B) 2 µg/mL.
  • IHC - NETO1 Antibody ASC11370
    Immunohistochemistry of NETO1 in rat lung tissue with NETO1 antibody at 2.5 µg/mL.
  • IF - NETO1 Antibody ASC11370
    Immunofluorescence of NETO1 in rat lung tissue with NETO1 antibody at 20 µg/mL.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession Q8TDF5
Other Accession NP_694821, 20452470
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application Notes NETO1 antibody can be used for detection of NETO1 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 81832
Target/Specificity NETO1; NETO1 antibody is predicted to not cross-react with other NETO protein family members.
Reconstitution & Storage NETO1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsNETO1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NETO1
Synonyms BTCL1
Function Involved in the development and/or maintenance of neuronal circuitry. Accessory subunit of the neuronal N-methyl-D- aspartate receptor (NMDAR) critical for maintaining the abundance of GRIN2A-containing NMDARs in the postsynaptic density. Regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, at least in the context of spatial learning and memory (By similarity).
Cellular Location Isoform 2: Cell membrane; Single-pass type I membrane protein. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Note=Component of the postsynaptic density (PSD) of excitatory synapses. Isoform 1: Secreted.
Tissue Location Isoform 1 and isoform 2 are retina-specific. Isoform 3 is found in retina as well as at lower levels in adult and fetal brain. EMBL; AF448838; AAM18026.1; -; mRNA EMBL; AF448839; AAM18027.1; -; mRNA EMBL; AC023301; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; AC091138; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; BC050329; AAH50329.1; -; mRNA EMBL; AL834354; CAD39019.1; -; mRNA CCDS; CCDS12000.1; -. [Q8TDF5-3] CCDS; CCDS42444.1; -. [Q8TDF5-1] RefSeq; NP_001188394.1; NM_001201465.1 RefSeq; NP_620416.1; NM_138966.3 RefSeq; NP_620552.1; NM_138999.1. [Q8TDF5-1] RefSeq; XP_005266831.1; XM_005266774.4 RefSeq; XP_016881506.1; XM_017026017.1. [Q8TDF5-3] RefSeq; XP_016881507.1; XM_017026018.1. [Q8TDF5-3] UniGene; Hs.465407; - ProteinModelPortal; Q8TDF5; - SMR; Q8TDF5; - BioGrid; 123590; 3 STRING; 9606.ENSP00000313088; - TCDB; 8.A.47.1.1; the neuropilin and tolloid-like (neto) family iPTMnet; Q8TDF5; - PhosphoSitePlus; Q8TDF5; - BioMuta; NETO1; - DMDM; 334302837; - PaxDb; Q8TDF5; - PeptideAtlas; Q8TDF5; - PRIDE; Q8TDF5; - ProteomicsDB; 74273; - ProteomicsDB; 74274; -. [Q8TDF5-1] ProteomicsDB; 74275; -. [Q8TDF5-2] Ensembl; ENST00000327305; ENSP00000313088; ENSG00000166342. [Q8TDF5-3] Ensembl; ENST00000397929; ENSP00000381024; ENSG00000166342. [Q8TDF5-1] Ensembl; ENST00000583169; ENSP00000464312; ENSG00000166342. [Q8TDF5-3] GeneID; 81832; - KEGG; hsa:81832; - UCSC; uc002lkw.3; human. [Q8TDF5-3] CTD; 81832; - DisGeNET; 81832; - EuPathDB; HostDB:ENSG00000166342.18; - GeneCards; NETO1; - HGNC; HGNC:13823; NETO1 HPA; HPA064630; - MIM; 607973; gene neXtProt; NX_Q8TDF5; - OpenTargets; ENSG00000166342; - PharmGKB; PA31558; - eggNOG; ENOG410IJXA; Eukaryota eggNOG; ENOG410ZS19; LUCA GeneTree; ENSGT00760000119018; - HOGENOM; HOG000060303; - HOVERGEN; HBG052600; - InParanoid; Q8TDF5; - OMA; MKHNYSQ; - OrthoDB; EOG091G060O; - PhylomeDB; Q8TDF5; - GenomeRNAi; 81832; - PRO; PR:Q8TDF5; - Proteomes; UP000005640; Chromosome 18 Bgee; ENSG00000166342; - CleanEx; HS_NETO1; - ExpressionAtlas; Q8TDF5; baseline and differential Genevisible; Q8TDF5; HS GO; GO:0030054; C:cell junction; IEA:UniProtKB-KW GO; GO:0060076; C:excitatory synapse; ISS:UniProtKB GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW GO; GO:0014069; C:postsynaptic density; ISS:UniProtKB GO; GO:0098839; C:postsynaptic density membrane; ISS:SynGO GO; GO:0035255; F:ionotropic glutamate receptor binding; IEA:Ensembl GO; GO:0007613; P:memory; ISS:UniProtKB GO; GO:2000463; P:positive regulation of excitatory postsynaptic potential; IEA:Ensembl GO; GO:0097120; P:receptor localization to synapse; IEA:Ensembl GO; GO:2000312; P:regulation of kainate selective glutamate receptor activity; IDA:SynGO GO; GO:0048169; P:regulation of long-term neuronal synaptic plasticity; ISS:UniProtKB GO; GO:0008542; P:visual learning; ISS:UniProtKB CDD; cd00041; CUB; 2 CDD; cd00112; LDLa; 1 Gene3D; 2.60.120.290; -; 2 InterPro; IPR000859; CUB_dom InterPro; IPR036055; LDL_receptor-like_sf InterPro; IPR023415; LDLR_class-A_CS InterPro; IPR002172; LDrepeatLR_classA_rpt InterPro; IPR028867; NETO1 InterPro; IPR035914; Sperma_CUB_dom_sf PANTHER; PTHR44143:SF1; PTHR44143:SF1; 1 Pfam; PF00431; CUB; 2 Pfam; PF00057; Ldl_recept_a; 1 SMART; SM00042; CUB; 2 SMART; SM00192; LDLa; 1 SUPFAM; SSF49854; SSF49854; 2 SUPFAM; SSF57424; SSF57424; 1 PROSITE; PS01180; CUB; 2 PROSITE; PS01209; LDLRA_1; 1 PROSITE; PS50068; LDLRA_2; 1 2: Evidence at transcript level; Alternative splicing; Cell junction; Cell membrane; Complete proteome; Disulfide bond; Glycoprotein; Membrane; Phosphoprotein; Polymorphism; Postsynaptic cell membrane; Receptor; Reference proteome; Repeat; Secreted; Signal; Synapse; Transmembrane; Transmembrane helix SIGNAL 1 22 CHAIN 23 533 Neuropilin and tolloid-like protein 1 /FTId=PRO_0000021799 TOPO_DOM 23 344 Extracellular. TRANSMEM 345 365 Helical. TOPO_DOM 366 533 Cytoplasmic. DOMAIN 41 155 CUB 1. {ECO:0000255|PROSITE- ProRule:PRU00059} DOMAIN 172 287 CUB 2. {ECO:0000255|PROSITE- ProRule:PRU00059} DOMAIN 291 327 LDL-receptor class A {ECO:0000255|PROSITE-ProRule:PRU00124} MOTIF 531 533 PDZ-binding MOD_RES 417 417 Phosphotyrosine {ECO:0000250|UniProtKB:Q8R4I7} CARBOHYD 306 306 N-linked (GlcNAc...) asparagine CARBOHYD 340 340 N-linked (GlcNAc...) asparagine DISULFID 41 68 DISULFID 96 118 DISULFID 172 202 DISULFID 229 251 DISULFID 292 304 DISULFID 299 317 DISULFID 311 326 VAR_SEQ 1 10 MIHGRSVLHI -> MLAEPFPKV (in isoform 1 and isoform 2) /FTId=VSP_012853 VAR_SEQ 157 157 D -> E (in isoform 1) /FTId=VSP_012854 VAR_SEQ 158 533 Missing (in isoform 1) /FTId=VSP_012855 VARIANT 481 481 S -> N (in dbSNP:rs922999) /FTId=VAR_051230 VARIANT 487 487 A -> G (in dbSNP:rs17086286) /FTId=VAR_051231 CONFLICT 140 140 D -> A (in Ref. 3; AAH50329) SEQUENCE 533 AA; 60191 MW; 763EBE7BAF06EAC8 CRC64; MIHGRSVLHI VASLIILHLS GATKKGTEKQ TTSETQKSVQ CGTWTKHAEG GIFTSPNYPS KYPPDRECIY IIEAAPRQCI ELYFDEKYSI EPSWECKFDH IEVRDGPFGF SPIIGRFCGQ QNPPVIKSSG RFLWIKFFAD GELESMGFSA RYNFTPDPDF KDLGALKPLP ACEFEMGGSE GIVESIQIMK EGKATASEAV DCKWYIRAPP RSKIYLRFLD YEMQNSNECK RNFVAVYDGS SSVEDLKAKF CSTVANDVML RTGLGVIRMW ADEGSRNSRF QMLFTSFQEP PCEGNTFFCH SNMCINNTLV CNGLQNCVYP WDENHCKEKR KTSLLDQLTN TSGTVIGVTS CIVIILIIIS VIVQIKQPRK KYVQRKSDFD QTVFQEVFEP PHYELCTLRG TGATADFADV ADDFENYHKL RRSSSKCIHD HHCGSQLSST KGSRSNLSTR DASILTEMPT QPGKPLIPPM NRRNILVMKH SYSQDAADAC DIDEIEEVPT TSHRLSRHDK AVQRFCLIGS LSKHESEYNT TRV
Research Areas
Citations (0)

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Background

NETO1 Antibody: Neuropilin and tolloid-like protein 1 (NETO1) is involved in the development and/or maintenance of neuronal circuitry. It is a type I transmembrane protein that is expressed in the brain and retina. NETO1 contains one LDL-receptor class A domain and two CUB domains and is either membrane-bound or secreted. It has three alternatively spliced isoforms, the first two of which are retina-specific and the third of which is found in both retina and brain tissue. Furthermore, as an accessory subunit of the neuronal N-methyl-D-aspartate receptor (NMDAR), it regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, at least in the context of spatial learning and memory.

References

Michishita M, Ikeda T, Nakashiba T, et al. A novel gene, Btcl1, encoding CUB and LDLa domains is expressed in restricted areas of mouse brain. Biochem. Biophys. Res. Commun. 2003; 306:680-6.
Stöhr H, Berger C, Fröhlich S, et al. A novel gene encoding a putative transmembrane protein with two extracellular CUB domains and a low-density lipoprotein class A module: isolation of alternatively spliced isoforms in retina and brain. Gene 2002; 286:223-31
Straub C, Hunt DL, Yamasaki M, et al. Distinct functions of kainate receptors in the brain are determined by the auxiliary subunit Neto1. Nat. Neurosci. 2011; 14:866-73.
Ng D, Pitcher GM, Szilard RK, et al. Neto1 is a novel CUB-domain NMDA receptor-interacting protein required for synaptic plasticity and learning. PLoS Biol. 2009; 7:e41

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Cat# ASC11370
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