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DPAGT1 Antibody

  • WB - DPAGT1 Antibody ASC11373
    Western blot analysis of DPAGT1 in mouse kidney tissue lysate with DPAGT1 antibody at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.
  • IHC - DPAGT1 Antibody ASC11373
    Immunohistochemistry of DPAGT1 in human kidney tissue with DPAGT1 antibody at 2.5 µg/mL.
  • IF - DPAGT1 Antibody ASC11373
    Immunofluorescence of DPAGT1 in human kidney tissue with DPAGT1 antibody at 20 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9H3H5
Other Accession NP_001373, 42794009
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 46090 Da
Application Notes DPAGT1 antibody can be used for detection of DPAGT1 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 1798
Target/Specificity DPAGT1; At least four isoforms of DPAGT1 are known to exist; this antibody will recognize the two longest isoforms. DPAGT1 antibody is predicted to not cross-react with UHRF1BP1.
Reconstitution & Storage DPAGT1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsDPAGT1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms DPAGT2
Function Catalyzes the initial step in the synthesis of dolichol- P-P-oligosaccharides.
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein
Citations (0)

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DPAGT1 Antibody: The UDP-N-acetylglucosamine-dolichyl-phosphate N-acetyl-glucosaminephosphotransferase (DPAGT1) is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. Mutations in this integral endoplasmic reticulum (ER) membrane protein enzyme belongs to the glycosyltransferase family 4 results in the congenital disorder of glycosylation type Ij with symptoms such as severe hypotonia, medically intractable seizures, mental retardation, microcephaly, and exotropia. Recent experiments have shown that DPAGT1 is a target of the Wnt/beta-catenin signaling pathway, with Wnt3a inducing higher DPAGT1 mRNA expression.


Wu X, Rush JS, Karaoglu D, et al. Deficiency of UDP-GlcNAc:Dolichol Phosphate N-Acetylglucosamine-1 Phosphate Transferase (DPAGT1) causes a novel congenital disorder of glycosylation type Ij. Hum. Mutat. 2003; 22:144-50.
Bretthauer RK. Structure, expression, and regulation of UDP-GlcNAc:dolichol phosphate GlcNAc-1-phosphate transferase (DPAGT1). Curr. Drug Targets 2009; 10:477-82
Sengupta PK, Bouchie MP, and Kukuruzinska MA. N-glycosylation gene DPAGT1 is a target of the Wnt/beta-catenin signaling pathway. J. Biol. Chem. 2010; 285:31164-73.

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$ 310.00
Cat# ASC11373
(40 western blots)
Availability: 5-7days
Bulk Size
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