|Application ||WB, IHC-P, E|
|Other Accession||NP_004870, 55956766|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||38965 Da|
|Application Notes||EI24 antibody can be used for detection of EI24 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL.|
|Target/Specificity||EI24; At least two isoforms of EI24 are known to exist; this antibody will only detect the larger isoform.|
|Reconstitution & Storage||EI24 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||EI24 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as a negative growth regulator via p53-mediated apoptosis pathway. Regulates formation of degradative autolysosomes during autophagy (By similarity).|
|Cellular Location||Nucleus membrane; Multi-pass membrane protein. Cytoplasm. Endoplasmic reticulum membrane; Multi-pass membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
EI24 Antibody: The etoposide-induced 24 (EI24) protein is an immediate-early induction target of p53-mediated apoptosis and functions as a tumor suppressor. EI24 contains six transmembrane domains and is localized to the endoplasmic reticulum (ER). It binds to Bcl-2 and may contribute to apoptosis by modulating the activity and function of Bcl-2. EI24 is highly mutated in aggressive breast cancers and its loss in breast cancer tissue correlates with tumor invasiveness. Similar findings in invasive cervical carcinoma suggest that EI24 may be a promising therapeutic target in the treatment of invasive cancer.
Lehar SM, Nacht M, Jacks T, et al. Identification and cloning of EI24, a gene induced by p53 in etoposide-treated cells. Oncogene 1996; 12:1181-7.
Gu Z, Flemington C, Chittenden T, et al. ei24, a p53 response gene involved in growth suppression and apoptosis. Mol. Cell Biol. 2000; 20:233-41
Zhao X, Ayer RE, Davis SL, et al. Apoptosis factor EI24/PIG8 is a novel endoplasmic reticulum-localized Bcl-2-binding protein which is associated with suppression of breast cancer invasiveness. Cancer Res. 2005; 65:2125-9.
Mazumder D, Mitra S, Singh RK, et al. Inactivation of CHEK1 and EI24 are associated with the development of invasive cervical carcinoma: clinical and prognostic implications. Int. J. Cancer 2010; epub
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