|Application ||WB, IHC-P, IF, E|
|Other Accession||BAG80561, 86990448|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||38650 Da|
|Application Notes||Neucrin antibody can be used for detection of Neucrin by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||C1orf187; At least three isoforms of Neucrin are known to exist; this antibody will detect the two shorter isoforms. Neucrin antibody is predicted to not cross-react with RILP.|
|Reconstitution & Storage||NEUCRIN antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||NEUCRIN Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Chemorepulsive axon guidance protein required for the development of spinal cord and forebrain commissures. Acts as a chemorepulsive guidance protein for commissural axons during development. Able to inhibit or repel neurite outgrowth from dorsal spinal cord. Inhibits the stabilization of cytosolic beta- catenin (CTNNB1) via its interaction with LRP6, thereby acting as an antagonist of Wnt signaling pathway.|
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Provided below are standard protocols that you may find useful for product applications.
NEUCRIN Antibody: Neucrin, also known as Draxin, is a repulsive guidance protein for the spinal cord and forebrain commissures. It is thought to act as an antagonist of canonical Wnt signaling by inhibiting the stabilization of cytosolic beta-catenin. Ectopically expressed neucrin inhibited growth or caused misrouting of chick spinal cord commissural axons in vivo while Neucrin-null mice showed defasciculation of spinal cord commissural axons and an absence of all forebrain commisures. Other experiments in mice have shown that olfactory bulb axonal outgrowth is inhibited by Neucrin, suggesting that Neucrin functions as an inhibitory guidance cue for olfactory bulb axons.
Islam SM, Shinmyo Y, Okafuji T, et al. Draxin, a repulsive guidance protein for spinal cord and forebrain commissures. Science 2009; 323:388-93.
Miyake A, Takahashi Y, Miwa H, et al. Neucrin is a novel neural-specific secreted antagonist to canonical Wnt signaling. Biochem. Biophys. Res. Commun. 2009; 390:1051-5.
Ahmed G, Shinmyo Y, Naser IB, et al. Olfactory bulb axonal outgrowth is inhibited by draxin. Biochem. Biophys. Res. Commun. 2010; 398:730-4.
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