|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_963925, 94981556|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||80778 Da|
|Application Notes||TGM5 antibody can be used for detection of TGM5 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||TGM5; TGM5 antibody is predicted to not cross-react with other TGase protein family members. Multiple isoforms of TGM5 are known to exist.|
|Reconstitution & Storage||TGM5 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||TGM5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Contributes to the formation of the cornified cell envelope of keratinocytes.|
|Cellular Location||Cytoplasm. Note=Associated with intermediate filaments|
|Tissue Location||Expressed in foreskin keratinocytes.|
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Provided below are standard protocols that you may find useful for product applications.
TGM5 Antibody: Transglutaminases (TGM) are a family of structurally and functionally related Ca2+-dependent enzymes (TGases) that stabilize protein assemblies through the formation of gamma-glutamyl-epsilon lysine crosslinks. TGases influence numerous biological processes, including blood coagulation, cell differentiation, fertilization and apoptosis. TGM5 belongs to the transglutaminase superfamily and catalyzes the cross-linking of proteins between glutamine and lysine residues, often resulting in stabilization of protein assemblies. TGM5 is also expressed in the human hair follicle. Defects in TGM5 are associated with acral peeling skin syndrome.
Ueki S, et al. Dual functions of transglutaminase in novel cell adhesion. J. Cell Sci. 1996; 109:2727-35.
Candi E, Oddi S, Terrinoni A, et al. Transglutaminase 5 cross-links loricrin, involicrin, and small proline-rich proteins in vitro. J. Biol. Chem. 2001; 276:35014-23.
Thibaut S, Candi E, Pietroni V, et al. Transglutaminase 5 expression in human hair follicle. J. Invest. Dermatol. 2005; 125:581-5
Cassidy AJ, van Steensel MA, Steijlen PM, et al. A homozygous missense mutation in TGM5 abolishes epidermal transglutaminase 5 activity and causes acral peeling skin syndrome. Am. J. Hum. Genet. 2005; 77:909-17.
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