|Application ||WB, ICC, E|
|Other Accession||NP_008932, 223029426|
|Calculated MW||36673 Da|
|Application Notes||SLC35D2 antibody can be used for detection of SLC35D2 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunocytochemistry starting at 5 µg/mL.|
|Target/Specificity||SLC35D2; At least two isoforms of SLC35D2 are known to exist; this antibody will recognize both isoforms. SLC35D2 antibody is predicted to not cross-react with SLC35D1 or SLC35D3.|
|Reconstitution & Storage||SLC35D2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||SLC35D2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Antiporter transporting nucleotide sugars such as UDP-N- acetylglucosamine (UDP-GlcNAc), UDP-glucose (UDP-Glc) and GDP- mannose (GDP-Man) pooled in the cytosol into the lumen of the Golgi in exchange for the corresponding nucleosides monophosphates (UMP for UDP-sugars and GMP for GDP-sugars). May take part in heparan sulfate synthesis by supplying UDP-Glc-NAc, the donor substrate, and thus be involved in growth factor signaling.|
|Cellular Location||Golgi apparatus membrane; Multi- pass membrane protein|
|Tissue Location||Highly expressed in heart, kidney, small intestine, placenta, lung and peripheral blood leukocyte. Weakly expressed in skeletal muscle and spleen. Not expressed in brain, colon and thymus.|
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Provided below are standard protocols that you may find useful for product applications.
SLC35D2 Antibody: The solute carrier family SLC35 consists of at least 17 proteins that act as nucleotide sugar transporters localized to the Golgi apparatus and endoplasmic reticulum. The role of the ER-resident SLC family member SLC35D2 is to transport both UDP-glucuronic acid and UDP-N-acetylgalactosamine. Its overexpression in transfected cells modulated cell surface heparin sulfate expression, suggesting that SLC35D2 is involved in heparin sulfate synthesis. SLC35D2-overexpressing cells also showed increased constitutive and hypotonic stress-stimulated release of UDP-GlcNAc, suggesting that SLC35D2 may be involved in UDP-sugar release and cell signaling.
Ishida N and Kawakita M. Molecular physiology and pathology of the nucleotide sugar transporter family (SLC35). Pflugers Arch. 2004; 447:768-75.
Suda T, Kamiyama S, Suzuki M, et al. Molecular cloning and characterization of a human multisubstrate specific nucleotide-sugar transporter homologous to Drosophila fringe connection. J. Biol. Chem. 2004; 279:26469-74
Sesma JI, Esther Jr CR, Kreda SM, et al. Endoplasmic reticulum/Golgi nucleotide sugar transporters contribute to the cellular release of UDP-sugar signaling molecules. J. Biol. Chem. 2009; 284:12572-83.
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