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SPATA1 Antibody

  • WB - SPATA1 Antibody ASC11393
    Western blot analysis of SPATA1 in A20 cell lysate with SPATA1 antibody at (A) 1 and (B) 2 µg/mL.
  • IHC - SPATA1 Antibody ASC11393
    Immunohistochemistry of SPATA1 in mouse testis tissue with SPATA1 antibody at 2.5 µg/mL.
  • IF - SPATA1 Antibody ASC11393
    Immunofluorescence of SPATA1 in mouse testis tissue with SPATA1 antibody at 20 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q5VX52
Other Accession Q5VX52, 341915102
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 50307 Da
Application Notes SPATA1 antibody can be used for detection of SPATA1 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 100505741
Target/Specificity SPATA1; At least two isoforms of SPATA1 are known to exist; this antibody will detect only the longest isoforms. SPATA1 antibody is predicted to not cross-react with other SPATA family members.
Reconstitution & Storage SPATA1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsSPATA1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Citations (0)

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SPATA1 Antibody: SPATA1, a novel cell-surface protein, is involved in shaping the sperm head during spermatogenesis. SPATA1 is localized at chromosome 1q21. At least three isoforms of SPATA1 are known to exist. The longest is preferentially expressed in hematopoietic tissues. Its extracellular domain contains a single immunoglobulin-like domain, and its intracellular segment has two immunoreceptor tyrosine-based inhibition motifs (ITIMs). It is thought to have an important role in hematopoietic cell signaling. The mid-sized isoform contains a short intracellular part without ITIMs, while the shortest isoform lacks the transmembrane segment and represents a potential soluble protein (2, 3).


Giesecke K, Hamann H, Stock KF, et al. Evaluation of SPATA1-associated markers for stallion fertility. Anim. Genet. 2009; 40:359-65.
Xu MJ, Zhao R, Zhao ZJ. Molecular cloning and characterization of SPAP1, an inhibitory receptor. Biochem. Biophys. Res. Commun. 2001; 280:768-75
Liu SF, He S, Liu BW, et al. Cloning and characterization of testis-specific spermatogenesis associated gene homologous to human SPATA4 in rat. Biol. Pharm. Bull. 2004; 27:1867-70.

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$ 310.00
Cat# ASC11393
(40 western blots)
Availability: 5-7days
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