|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_055134, 7706607|
|Calculated MW||99997 Da|
|Application Notes||LEMD3 antibody can be used for detection of LEMD3 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 5 µg/mL.|
|Target/Specificity||LEMD3; At least three isoforms of LEMD3 are known to exist; this antibody will detect all isoforms. LEMD3 antibody is predicted to not cross-react with LEMD1 and LEMD2|
|Reconstitution & Storage||LEMD3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||LEMD3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Can function as a specific repressor of TGF-beta, activin, and BMP signaling through its interaction with the R-SMAD proteins. Antagonizes TGF-beta-induced cell proliferation arrest.|
|Cellular Location||Nucleus inner membrane; Multi-pass membrane protein|
|Tissue Location||Heart, brain, placenta, lung, liver and skeletal muscle|
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Provided below are standard protocols that you may find useful for product applications.
LEMD3 Antibody: LEMD3 (LEM domain-containing 3) is a lem-domain containing protein, also known as MAN1, whose loss of function results in disorders characterized by osteopoikilosis, Buschke-Ollendorff syndrome, and melorheostosis. LEMD3 is an inner nuclear membrane protein that interacts with BMP and activin-TGF-beta receptor-activated Smads and antagonizes TGF-beta signaling in human cells.
Hellemans J, Preobrazhenska O, Willaert A, et al. Loss-of-function mutations in LEMD3 result in osteopoikilosis, Buschke-Ollendorff syndrome, and melorheostosis. Nat. Genet. 2004; 36:1213-8.
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