|Application ||WB, E|
|Other Accession||AAH43157, 148664230|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||104114 Da|
|Application Notes||ANKLE2 antibody can be used for detection of ANKLE2 by Western blot at 1 - 2 µg/mL.|
|Target/Specificity||ANKLE2; Three isoforms of ANKLE2 are known to exist; this antibody will detect all three. ANKLE2 antibody is predicted to not cross-react with ANKLE1.|
|Reconstitution & Storage||ANKLE2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||ANKLE2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in mitotic nuclear envelope reassembly by promoting dephosphorylation of BAF/BANF1 during mitotic exit. Coordinates the control of BAF/BANF1 dephosphorylation by inhibiting VRK1 kinase and promoting dephosphorylation of BAF/BANF1 by protein phosphatase 2A (PP2A), thereby facilitating nuclear envelope assembly. It is unclear whether it acts as a real PP2A regulatory subunit or whether it is involved in recruitment of the PP2A complex. Involved in brain development (PubMed:25259927).|
|Cellular Location||Endoplasmic reticulum membrane; Single-pass type III membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
ANKLE2 Antibody: Ankyrins are membrane adaptor molecules that play important roles in coupling integral membrane proteins to the spectrin-based cytoskeleton network. ANKLE2 (Ankyrin repeat and LEM domain containing 2) is a member of ankyrin repeat and LEM domain containing family. It is a potential single-pass membrane protein that contains three ankyrin repeats and a Lem domain. Ankyrin repeats mediate protein-protein interactions and are among the most common structural motifs in known proteins. The function of this protein is still under investigation.
Lee KK and Wilson KL. All in the family: evidence for four new LEM-domain proteins Lem2 (NET-25), Lem3, Lem4 and Lem5 in the human genome. Symp. Soc. Exp. Biol. 2004; 56:329-39
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